Microbial nitrogen limitation in the mammalian large intestine.

Autor: Reese AT; Department of Biology, Duke University, Durham, NC, USA.; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA., Pereira FC; Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, Austria., Schintlmeister A; Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, Austria.; Large-Instrument Facility for Advanced Isotope Research, Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, Austria., Berry D; Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, Austria., Wagner M; Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, Austria.; Large-Instrument Facility for Advanced Isotope Research, Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, Austria., Hale LP; Department of Pathology, Duke University Medical Center, Durham, NC, USA., Wu A; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA., Jiang S; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA., Durand HK; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA., Zhou X; Department of Medicine, Duke University Medical Center, Durham, NC, USA., Premont RT; Department of Medicine, Duke University Medical Center, Durham, NC, USA., Diehl AM; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA.; Department of Medicine, Duke University Medical Center, Durham, NC, USA., O'Connell TM; Department of Otolaryngology - Head & Neck Surgery, Indiana University School of Medicine, Indianapolis, IN, USA., Alberts SC; Department of Biology, Duke University, Durham, NC, USA.; Department of Evolutionary Anthropology, Duke University, Durham, NC, USA.; Institute of Primate Research, National Museums of Kenya, Nairobi, Kenya., Kartzinel TR; Department of Ecology and Evolutionary Biology, Brown University, Providence, RI, USA., Pringle RM; Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, USA., Dunn RR; Department of Applied Ecology, North Carolina State University, Raleigh, NC, USA., Wright JP; Department of Biology, Duke University, Durham, NC, USA., David LA; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC, USA. lawrence.david@duke.edu.; Center for Genomic and Computational Biology, Duke University, Durham, NC, USA. lawrence.david@duke.edu.
Jazyk: angličtina
Zdroj: Nature microbiology [Nat Microbiol] 2018 Dec; Vol. 3 (12), pp. 1441-1450. Date of Electronic Publication: 2018 Oct 29.
DOI: 10.1038/s41564-018-0267-7
Abstrakt: Resource limitation is a fundamental factor governing the composition and function of ecological communities. However, the role of resource supply in structuring the intestinal microbiome has not been established and represents a challenge for mammals that rely on microbial symbionts for digestion: too little supply might starve the microbiome while too much might starve the host. We present evidence that microbiota occupy a habitat that is limited in total nitrogen supply within the large intestines of 30 mammal species. Lowering dietary protein levels in mice reduced their faecal concentrations of bacteria. A gradient of stoichiometry along the length of the gut was consistent with the hypothesis that intestinal nitrogen limitation results from host absorption of dietary nutrients. Nitrogen availability is also likely to be shaped by host-microbe interactions: levels of host-secreted nitrogen were altered in germ-free mice and when bacterial loads were reduced via experimental antibiotic treatment. Single-cell spectrometry revealed that members of the phylum Bacteroidetes consumed nitrogen in the large intestine more readily than other commensal taxa did. Our findings support a model where nitrogen limitation arises from preferential host use of dietary nutrients. We speculate that this resource limitation could enable hosts to regulate microbial communities in the large intestine. Commensal microbiota may have adapted to nitrogen-limited settings, suggesting one reason why excess dietary protein has been associated with degraded gut-microbial ecosystems.
Databáze: MEDLINE
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