Adhesion and biofilm formation of Candida parapsilosis isolated from vaginal secretions to copper intrauterine devices.

Autor:	Borges KRA; Universidade Federal do Maranhão, Programa de Pós-Graduação em Saúde do Adulto, São Luís, Maranhão, Brazil., Pimentel IV; Universidade Federal do Maranhão, Programa de Pós-Graduação em Saúde do Adulto, São Luís, Maranhão, Brazil., Lucena LCLDS; Universidade Ceuma, Curso de Enfermagem, São Luís, Maranhão, Brazil., Silva MACND; Universidade Estadual de Campinas, Programa de Pós-Graduação em Clínica Médica, Campinas, São Paulo, Brazil., Monteiro SG; Universidade Ceuma, Programa de Pós-Graduação em Biologia Parasitária, São Luís, Maranhão, Brazil., Monteiro CA; Universidade Ceuma, Programa de Pós-Graduação em Biologia Parasitária, São Luís, Maranhão, Brazil., Nascimento MDDSB; Universidade Federal do Maranhão, Programa de Pós-Graduação em Saúde do Adulto, São Luís, Maranhão, Brazil., Bezerra GFB; Universidade Federal do Maranhão, Programa de Pós-Graduação em Saúde do Adulto, São Luís, Maranhão, Brazil.
Jazyk:	angličtina
Zdroj:	Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2018 Oct 22; Vol. 60, pp. e59. Date of Electronic Publication: 2018 Oct 22.
DOI:	10.1590/S1678-9946201860059
Abstrakt:	Introduction: Candida parapsilosis is one of the main species that is able to adhere to forming biofilms on inert materials. Adhesion is the first step towards the colonization and invasion of host cells during the infectious process. Among the infections, vulvovaginal candidiasis is increasingly common. The objective was to evaluate the profile of adherence and biofilm formation of eight isolates of C. parapsilosis on the metal used in intrauterine devices (IUDs). Methods: Eight strains of C. parapsilosis presenting strong adhesion and biofilm formation properties were isolated from vaginal secretions in a previous study. To assay the adhesion and biofilm formation, copper fragments were made and cultivated in tubes containing 3 mL of phosphate-buffered saline and incubated for 6 and 24 h at 37 °C to evaluate biofilm formation. After incubation, the intensity of adherence and of biofilm formation on copper fragments were determined by performing a colony count. Results: All isolates were able to form biofilms and the isolate Cp62 showed many cells joined in a planktonic mode forming biofilms. The use of an IUD is one of the main factors that favors vulvovaginal candidiasis, and the presence of copper in this device increases the chance of recurrent vulvovaginal candidiasis (CVVR) due to the ease with which species of the genus Candida can adhere to inert surfaces. Conclusion: This research showed that the clinical isolates studied adhered to IUD copper fragments and formed biofilms, further increasing their virulence.
Databáze:	MEDLINE
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