Persistence of radiation-induced aberrations in patients after radiotherapy with C-ions and IMRT.

Autor:	Hartel C; GSI Helmholtzzentrum fuer Schwerionenforschung, Biophysics Department, Darmstadt, Germany., Nasonova E; GSI Helmholtzzentrum fuer Schwerionenforschung, Biophysics Department, Darmstadt, Germany.; Joint Institute for Nuclear Research (JINR), Laboratory of Radiation Biology, Dubna, Russia., Fuss MC; GSI Helmholtzzentrum fuer Schwerionenforschung, Biophysics Department, Darmstadt, Germany., Nikoghosyan AV; University Heidelberg, Department of Radiation Oncology, Germany., Debus J; University Heidelberg, Department of Radiation Oncology, Germany., Ritter S; GSI Helmholtzzentrum fuer Schwerionenforschung, Biophysics Department, Darmstadt, Germany.
Jazyk:	angličtina
Zdroj:	Clinical and translational radiation oncology [Clin Transl Radiat Oncol] 2018 Oct 10; Vol. 13, pp. 57-63. Date of Electronic Publication: 2018 Oct 10 (Print Publication: 2018).
DOI:	10.1016/j.ctro.2018.10.002
Abstrakt:	Background and Purpose: Chromosomal aberrations in peripheral blood lymphocytes are a biomarker for radiation exposure and are associated with an increased risk for malignancies. To determine the long-term cytogenetic effect of radiotherapy, we analyzed the persistence of different aberration types up to 2.5 years after the treatment. Materials and Methods: Cytogenetic damage was analyzed in lymphocytes from 14 patients that had undergone C-ion boost + IMRT treatment for prostate cancer. Samples were taken immediately, 1 year and 2.5 years after therapy. Aberrations were scored using the multiplex fluorescence in situ hybridization technique and grouped according to their transmissibility to daughter cells. Results: Dicentric chromosomes (non-transmissible) and translocations (transmissible) were induced with equal frequencies. In the follow-up period, the translocation yield remained unchanged while the yield of dicentrics decreased to ≈40% of the initial value (p = 0.011 and p = 0.001 for 1 and 2.5 years after compared to end of therapy). In 2 patients clonal aberrations were observed; however they were also found in samples taken before therapy and thus were not radiotherapy induced. Conclusion: The shift in the aberrations spectrum towards a higher fraction of translocations indicates the exposure of hematopoietic stem and progenitor cells underlining the importance of a careful sparing of bone marrow during radiotherapy to minimize the risk for secondary cancers.
Databáze:	MEDLINE
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