| Autor: |
Mareque C; Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológica Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay., da Silva TF; Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Vollú RE; Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Beracochea M; Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológica Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay., Seldin L; Laboratório de Genética Microbiana, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, Brazil., Battistoni F; Departamento de Bioquímica y Genómica Microbianas, Instituto de Investigaciones Biológica Clemente Estable, Avenida Italia 3318, Montevideo 11600, Uruguay. |
| Abstrakt: |
Sweet sorghum ( Sorghum bicolor ) is a multipurpose crop used as a feedstock to produce bioethanol, sugar, energy, and animal feed. However, it requires high levels of N fertilizer application to achieve the optimal growth, which causes environmental degradation. Bacterial endophytes, which live inside plant tissues, play a key role in the health and productivity of their host. This particular community may be influenced by different agronomical practices. The aim of the work was to evaluate the effects of N fertilization on the structure, diversity, abundance, and composition of endophytic and diazotrophic bacterial community associated with field-grown sweet sorghum. PCR-DGGE, quantitative PCR, and high-throughput sequencing were performed based on the amplification of rrs and nifH genes. The level of N fertilization affected the structure and abundance but not the diversity of the endophytic bacterial communities associated with sweet sorghum plants. This effect was pronounced in the roots of both bacterial communities analyzed and may depend on the physiological state of the plants. Specific bacterial classes and genera increased or decreased when the fertilizer was applied. The data obtained here contribute to a better understanding on the effects of agronomical practices on the microbiota associated with this important crop, with the aim to improve its sustainability. |
