Cafeteria Diet Feeding in Young Rats Leads to Hepatic Steatosis and Increased Gluconeogenesis under Fatty Acids and Glucagon Influence.

Autor: Maeda Júnior AS; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. jrmaedaopcao@gmail.com., Constantin J; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. jconstantin@uem.br., Utsunomiya KS; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. ksutsunomiya2@uem.br., Gilglioni EH; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. gilglioni@hotmail.com., Gasparin FRS; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. fabygasparin@hotmail.com., Carreño FO; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. fer.carreno@gmail.com., de Moraes SMF; Department of Morphophysiological Sciences, State University of Maringá, Maringá 87020-900, Paraná, Brazil. smfmoraes@uem.br., Rocha M; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. marciorocha@mail.com., Natali MRM; Department of Morphophysiological Sciences, State University of Maringá, Maringá 87020-900, Paraná, Brazil. mrmnatali@uem.br., Ghizoni CVC; Department of Biochemistry, Laboratory of Liver Metabolism, State University of Maringá, Maringá 87020-900, Paraná, Brazil. crisvizioli@gmail.com., Bracht A; Department of Biochemistry, Laboratory of Liver Metabolism, State University of Maringá, Maringá 87020-900, Paraná, Brazil. adebracht@uol.com.br., Ishii-Iwamoto EL; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. eliiwamoto@uem.br., Constantin RP; Department of Biochemistry, Laboratory of Biological Oxidations and Laboratory of Experimental Steatosis, State University of Maringá, Maringá 87020-900, Paraná, Brazil. rpconstantin@uem.br.
Jazyk: angličtina
Zdroj: Nutrients [Nutrients] 2018 Oct 23; Vol. 10 (11). Date of Electronic Publication: 2018 Oct 23.
DOI: 10.3390/nu10111571
Abstrakt: Gluconeogenesis overstimulation due to hepatic insulin resistance is the best-known mechanism behind elevated glycemia in obese subjects with hepatic steatosis. This suggests that glucose production in fatty livers may differ from that of healthy livers, also in response to other gluconeogenic determinant factors, such as the type of substrate and modulators. Thus, the aim of this study was to investigate the effects of these factors on hepatic gluconeogenesis in cafeteria diet-induced obese adult rats submitted to a cafeteria diet at a young age. The livers of the cafeteria group exhibited higher gluconeogenesis rates when glycerol was the substrate, but lower rates were found when lactate and pyruvate were the substrates. Stearate or glucagon caused higher stimulations in gluconeogenesis in cafeteria group livers, irrespective of the gluconeogenic substrates. An increased mitochondrial NADH/NAD⁺ ratio and a reduced rate of 14 CO₂ production from [ 14 C] fatty acids suggested restriction of the citric acid cycle. The higher glycogen and lipid levels were possibly the cause for the reduced cellular and vascular spaces found in cafeteria group livers, likely contributing to oxygen consumption restriction. In conclusion, specific substrates and gluconeogenic modulators contribute to a higher stimulation of gluconeogenesis in livers from the cafeteria group.
Databáze: MEDLINE
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