The Genome Sequence of the Wild Tomato Solanum pimpinellifolium Provides Insights Into Salinity Tolerance.

Autor:	Razali R; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Bougouffa S; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Morton MJL; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Lightfoot DJ; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Alam I; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.; Division of Computer, Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Essack M; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Arold ST; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Kamau AA; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.; Division of Computer, Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Schmöckel SM; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Pailles Y; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Shahid M; International Center for Biosaline Agriculture, Dubai, United Arab Emirates., Michell CT; Red Sea Research Center, Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Al-Babili S; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Ho YS; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Tester M; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Bajic VB; Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.; Division of Computer, Electrical and Mathematical Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia., Negrão S; Division of Biological and Environmental Sciences and Engineering, The Bioactives Lab, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2018 Oct 04; Vol. 9, pp. 1402. Date of Electronic Publication: 2018 Oct 04 (Print Publication: 2018).
DOI:	10.3389/fpls.2018.01402
Abstrakt:	Solanum pimpinellifolium , a wild relative of cultivated tomato, offers a wealth of breeding potential for desirable traits such as tolerance to abiotic and biotic stresses. Here, we report the genome assembly and annotation of S. pimpinellifolium 'LA0480.' Moreover, we present phenotypic data from one field experiment that demonstrate a greater salinity tolerance for fruit- and yield-related traits in S. pimpinellifolium compared with cultivated tomato. The 'LA0480' genome assembly size (811 Mb) and the number of annotated genes (25,970) are within the range observed for other sequenced tomato species. We developed and utilized the Dragon Eukaryotic Analyses Platform (DEAP) to functionally annotate the 'LA0480' protein-coding genes. Additionally, we used DEAP to compare protein function between S. pimpinellifolium and cultivated tomato. Our data suggest enrichment in genes involved in biotic and abiotic stress responses. To understand the genomic basis for these differences in S. pimpinellifolium and S. lycopersicum , we analyzed 15 genes that have previously been shown to mediate salinity tolerance in plants. We show that S. pimpinellifolium has a higher copy number of the inositol-3-phosphate synthase and phosphatase genes, which are both key enzymes in the production of inositol and its derivatives. Moreover, our analysis indicates that changes occurring in the inositol phosphate pathway may contribute to the observed higher salinity tolerance in 'LA0480.' Altogether, our work provides essential resources to understand and unlock the genetic and breeding potential of S. pimpinellifolium , and to discover the genomic basis underlying its environmental robustness.
Databáze:	MEDLINE
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