Virome Analysis Reveals No Association of Head and Neck Vascular Anomalies with an Active Viral Infection.

Autor:	Franke N; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Bette M; Institute of Anatomy and Cell Biology, Philipps University, Marburg, Germany., Marquardt A; Department of Hematology, Oncology and Immunology, University Hospital Giessen and Marburg, Marburg, Germany., Briese T; Center for Infection and Immunity, Columbia University, New York City, NY, U.S.A., Lipkin WI; Center for Infection and Immunity, Columbia University, New York City, NY, U.S.A., Kurz C; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Ehrenreich J; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Mack E; Department of Hematology, Oncology and Immunology, University Hospital Giessen and Marburg, Marburg, Germany., Baying B; Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany., Beneš V; Genomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany., Rodepeter FR; Institute of Pathology, Philipps University, Marburg, Germany., Neff A; Department of Oral and Maxillofacial Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Teymoortash A; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Eivazi B; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Geisthoff U; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Stuck BA; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany., Bakowsky U; Department of Pharmaceutical Technology and Biopharmacy, Philipps University, Marburg, Germany., Mandic R; Department of Otolaryngology/Head and Neck Surgery, University Hospital Giessen and Marburg, Marburg, Germany mandic@med.uni-marburg.de.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2018 Nov-Dec; Vol. 32 (6), pp. 1323-1331.
DOI:	10.21873/invivo.11382
Abstrakt:	Background/aim: Vascular anomalies encompass different vascular malformations [arteriovenous (AVM), lymphatic (LM), venous lymphatic (VLM), venous (VM)] and vascular tumors such as hemangiomas (HA). The pathogenesis of vascular anomalies is still poorly understood. Viral infection was speculated as a possible underlying cause. Materials and Methods: A total of 13 human vascular anomalies and three human skin control tissues were used for viral analysis. RNA derived from AVM (n=4) and normal skin control (n=3) tissues was evaluated by RNA sequencing. The Virome Capture Sequencing Platform for Vertebrate Viruses (VirCapSeq-VERT) was deployed on 10 tissues with vascular anomalies (2×AVM, 1×HA, 1×LM, 2×VLM, 4×VM). Results: RNA sequencing did not show any correlation of AVM with viral infection. By deploying VirCapSeq-VERT, no consistent viral association was seen in the tested tissues. Conclusion: The analysis does not point to the presence of an active viral infection in vascular anomalies. However, transient earlier viral infections, e.g. during pregnancy, cannot be excluded with this approach. (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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