Comparison of semen characteristics and sperm cryopreservation in common marmoset (Callithrix jacchus) and black-tufted-ear marmoset (Callithrix penicillata).

Autor: Arakaki PR; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil., Nichi M; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil., Monteiro FOB; Postgraduate Program in Animal Health and Production in Amazonia, Federal Rural University of the Amazon, Belém, PA, Brazil., Muniz JAPC; National Primate Center, Health Ministry, Ananindeua, PA, Brazil., Guimarães MABV; Department of Animal Reproduction, College of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil., Valle RDRD; Institute of Health Sciences, Paulista University, São Paulo, SP, Brazil.
Jazyk: angličtina
Zdroj: Journal of medical primatology [J Med Primatol] 2019 Feb; Vol. 48 (1), pp. 32-42. Date of Electronic Publication: 2018 Oct 21.
DOI: 10.1111/jmp.12388
Abstrakt: Background: The common marmoset (Callithrix jacchus) is one of the most studied Neotropical primates regarding reproduction. However, little has been reported on the black-tufted-ear marmoset (Callithrix penicillata), which may produce fertile hybrids with other Callithrix. This is the first study to evaluate sperm freezing from black-tufted-ear marmoset.
Methods: Testicles from all animals were measured, and semen was collected by penile vibrostimulation. Samples were analyzed after collection, after chilling and addition of glycerol, and after thawing.
Results and Conclusions: Fresh semen from both species was similar in many aspects. Additionally, there is a relationship between total motility, plasma membrane integrity, and acrosome integrity observed in sperm samples from both species. We managed to evaluate fresh and thawed sperm with suitable methods for use under zoo or field conditions. However, TEST egg yolk with glycerol at 4% and 6% concentration was not effective for sperm protection in both species during cryopreservation.
(© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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