Characterization of Panton-Valentine leukocidin-positive Staphylococcus aureus from skin and soft tissue infections and wounds in Nigeria: a cross-sectional study.
| Autor: | Ayepola OO; Department of Biological Sciences, Covenant University, Ota, Ogun, Nigeria., Olasupo NA; Department of Microbiology, Lagos State University, Ojo, Lagos, Nigeria., Egwari LO; Department of Biological Sciences, Covenant University, Ota, Ogun, Nigeria., Schaumburg F; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | F1000Research [F1000Res] 2018 Jul 30; Vol. 7, pp. 1155. Date of Electronic Publication: 2018 Jul 30 (Print Publication: 2018). |
| DOI: | 10.12688/f1000research.15484.1 |
| Abstrakt: | Background: Staphylococcus aureus is a significant pathogen implicated in numerous nosocomial and community-acquired infections. The Panton-Valentine leukocidin (PVL) can be associated with severe necrotizing diseases such as pneumonia, skin and soft tissue infection (SSTI). Methods : In total, 96 S. aureus isolates were obtained from patients presenting with wounds (n=48) and soft tissue infections (SSTIs, n=48). These were characterized based on their antimicrobial susceptibility profile, the possession of virulence genes (e.g. capsular type, PVL), accessory gene regulator ( agr ) type, and the staphylococcal protein A ( spa ) type. The production of the PVL protein was assessed by western blotting. Results : All isolates were susceptible to methicillin. The resistance was highest to penicillin (97.9%), followed by trimethoprim/sulfamethoxazole (85.4%) and tetracycline (10.4%). The PVL gene was found in 83.3% of isolates from SSTIs and in 79.2% of isolates from wound. Of these, 53 (68%) produced PVL as assessed by western blotting. The most prevalent spa type was the t084 (78.1%, n=75) and, majority of the isolates carried agr 2 (82.3%, n=79). Conclusions : Prevalence of antibiotic resistant PVL-positive methicillin susceptible S. aureus strains has severe implications on PVL mediated infections. Competing Interests: No competing interests were disclosed. |
| Databáze: | MEDLINE |
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