Modulation of plant and bacterial polyamine metabolism during the compatible interaction between tomato and Pseudomonas syringae.

Autor:	Vilas JM; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Romero FM; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Rossi FR; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Marina M; Laboratorio de fisiología y bioquímica de la maduración de frutos, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Maiale SJ; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Calzadilla PI; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Pieckenstain FL; Laboratorio de interacciones planta-microorganismo, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Ruiz OA; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina., Gárriz A; Laboratorio de estrés biótico y abiótico en plantas, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de San Martín (UNSAM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Avenida Intendente Marino Km 8.200 CC 164 (7130), Chascomús, Buenos Aires, Argentina. Electronic address: garriz@intech.gov.ar.
Jazyk:	angličtina
Zdroj:	Journal of plant physiology [J Plant Physiol] 2018 Dec; Vol. 231, pp. 281-290. Date of Electronic Publication: 2018 Sep 27.
DOI:	10.1016/j.jplph.2018.09.014
Abstrakt:	The polyamines putrescine, spermidine and spermine participate in a variety of cellular processes in all organisms. Many studies have shown that these polycations are important for plant immunity, as well as for the virulence of diverse fungal phytopathogens. However, the polyamines' roles in the pathogenesis of phytopathogenic bacteria have not been thoroughly elucidated to date. To obtain more information on this topic, we assessed the changes in polyamine homeostasis during the infection of tomato plants by Pseudomonas syringae. Our results showed that polyamine biosynthesis and catabolism are activated in both tomato and bacteria during the pathogenic interaction. This activation results in the accumulation of putrescine in whole leaf tissues, as well as in the apoplastic fluids, which is explained by the induction of its synthesis in plant cells and also on the basis of its excretion by bacteria. We showed that the excretion of this polyamine by P. syringae is stimulated under virulence-inducing conditions, suggesting that it plays a role in plant colonization. However, no activation of bacterial virulence traits or induction of plant invasion was observed after the exogenous addition of putrescine. In addition, no connection was found between this polyamine and plant defence responses. Although further research is warranted to unravel the biological functions of these molecules during plant-bacterial interactions, this study contributes to a better understanding of the changes associated with the homeostasis of polyamines during plant pathogenesis. (Copyright © 2018 Elsevier GmbH. All rights reserved.)
Databáze:	MEDLINE
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