Label-free independent quantitation of viable and non-viable cells using a multivariable multi-resonant sensor.

Autor: Potyrailo RA; GE Global Research, Niskayuna, NY 12309, USA. Electronic address: potyrailo@ge.com., Dieringer J; GE Global Research, Niskayuna, NY 12309, USA., Cotero V; GE Global Research, Niskayuna, NY 12309, USA., Lee Y; GE Global Research, Niskayuna, NY 12309, USA., Go S; GE Global Research, Niskayuna, NY 12309, USA., Schulmerich M; GE Global Research, Niskayuna, NY 12309, USA., Malmquist G; GE Healthcare Life Sciences, 751 84 Uppsala, Sweden., Castan A; GE Healthcare Life Sciences, 751 84 Uppsala, Sweden., Gebauer K; GE Healthcare Life Sciences, 751 84 Uppsala, Sweden., Pizzi V; GE Healthcare Life Sciences, Marlborough, MA 01752, USA.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2019 Feb; Vol. 125, pp. 97-104. Date of Electronic Publication: 2018 Oct 10.
DOI: 10.1016/j.bioelechem.2018.10.001
Abstrakt: Biological cells are utilized for diverse biotechnological and bioengineering purposes ranging from the production of biopharmaceuticals, to cell therapy, "human-on-a-chip" drug and toxicology assays, and drug-resistance tests. In these and other applications, it is critical to quantify the levels of not only viable but also non-viable cells. While traditional off-line cell-staining methods are available for counting of non-viable cells, many applications cannot periodically remove cells for their off-line analysis because of the risk of contamination or workflow logistics. Here we show in-situ label-free quantitation of viable and non-viable cells with multivariable multi-resonant sensors. We used Chinese hamster ovary (CHO) cells in suspension culture in single-use bioreactors as a representative example. The resonant sensor design strategy permitted enhanced sensor sensitivity versus conventional non-resonant measurements and probed the spectral dispersion of viable and non-viable cells with multiple resonances. These capabilities of label-free in-situ analysis of cell viability can be attractive in diverse cell applications such as cell suspensions, adhered cells, and their 3D assemblages.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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