| Autor: |
Leung HM; Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. hmhleung@hkbu.edu.hk.; Department of History, Hong Kong Shue Yan University, North Point, Hong Kong, China. hmhleung@hkbu.edu.hk.; Upper Iowa University, 605 Washington St, Fayette, IA, 52142, USA. hmhleung@hkbu.edu.hk., Kwok FSL; Department of Applied Sciences, Institute of Vocational Education, Hong Kong, China., Mo WY; Department of Science and Environmental Studies, The Hong Kong Education University, Tai Po, Hong Kong, China., Cheung KC; Department of Applied Sciences, Institute of Vocational Education, Hong Kong, China., Yue YK; Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China., Wong YK; School of Science and Technology, The Open University of Hong Kong, Ho Man Tin, Hong Kong., Au CK; Department of History, Hong Kong Shue Yan University, North Point, Hong Kong, China., Tsui MTK; Department of Biology, University of North Carolina at Greensboro, Greensboro, NC, 27402, USA., Yung KKL; Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. kklyung@hkbu.edu.hk. |
| Abstrakt: |
The objective of this research is to investigate the enzymatic activities between protein disulfide isomerase (PDI) found in animals and plants and the properties found in a commonly used Chinese medicine called Sijunzi Tang. During the investigation, PDI, which is a monomer with a molecular mass of 57.0 kDa, was used to reactivate malate dehydrogenase (MDH). However, with the interference of polycyclic aromatic hydrocarbons (PAHs), evidence indicates that such chemicals are carcinogenic, mutagenic, and toxic to humans. The enzymatic activity of PDI found in animal's liver and plant was 1657 folds of purification; 0.284 unit/mg of enzyme activity, and 5694.4 folds of purification; 1.00 unit/mg of enzyme activity, respectively. PDI extracted in treated animal and plant tissue revealed 2.40% and 80.44% of regaining MDH enzymatic activity, respectively. Although in its initial phase of investigation, it is assumed that the properties found in Sijunzi Tang can help regain enzymatic activity in those affected by xenobiotic substances, thus, making it a potential ingredient in assisting with PDI functions. |
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