White-nose syndrome is associated with increased replication of a naturally persisting coronaviruses in bats.

Autor: Davy CM; Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada.; Ontario Ministry of Natural Resources and Forestry, Wildlife Research and Monitoring Section, Trent University, Peterborough, ON, Canada., Donaldson ME; Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada., Subudhi S; Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada., Rapin N; Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada., Warnecke L; Department of Biology, University of Winnipeg, Manitoba, Canada.; Department of Animal Ecology and Conservation, University Hamburg, Hamburg, Hamburg, Germany., Turner JM; Department of Biology, University of Winnipeg, Manitoba, Canada.; Institute for Land Water and Society, Charles Sturt University, Albury, New South Wales, Australia., Bollinger TK; Canadian Wildlife Health Cooperative and Department of Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada., Kyle CJ; Forensic Science Department, Trent University, Peterborough, ON, Canada., Dorville NAS; Department of Biology, University of Winnipeg, Manitoba, Canada., Kunkel EL; Department of Biology, University of Winnipeg, Manitoba, Canada., Norquay KJO; Department of Biology, University of Winnipeg, Manitoba, Canada., Dzal YA; Department of Biology, University of Winnipeg, Manitoba, Canada., Willis CKR; Department of Biology, University of Winnipeg, Manitoba, Canada., Misra V; Department of Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. vikram.misra@usask.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Oct 19; Vol. 8 (1), pp. 15508. Date of Electronic Publication: 2018 Oct 19.
DOI: 10.1038/s41598-018-33975-x
Abstrakt: Spillover of viruses from bats to other animals may be associated with increased contact between them, as well as increased shedding of viruses by bats. Here, we tested the prediction that little brown bats (Myotis lucifugus) co-infected with the M. lucifugus coronavirus (Myl-CoV) and with Pseudogymnoascus destructans (Pd), the fungus that causes bat white-nose syndrome (WNS), exhibit different disease severity, viral shedding and molecular responses than bats infected with only Myl-CoV or only P. destructans. We took advantage of the natural persistence of Myl-CoV in bats that were experimentally inoculated with P. destructans in a previous study. Here, we show that the intestines of virus-infected bats that were also infected with fungus contained on average 60-fold more viral RNA than bats with virus alone. Increased viral RNA in the intestines correlated with the severity of fungus-related pathology. Additionally, the intestines of bats infected with fungus exhibited different expression of mitogen-activated protein kinase pathway and cytokine related transcripts, irrespective of viral presence. Levels of coronavirus antibodies were also higher in fungal-infected bats. Our results suggest that the systemic effects of WNS may down-regulate anti-viral responses in bats persistently infected with M. lucifugus coronavirus and increase the potential of virus shedding.
Databáze: MEDLINE