The link between nitrous oxide emissions, microbial community profile and function from three full-scale WWTPs.

Autor: Vieira A; iBET - Instituto de Biologia Experimental e Tecnológica, Av. República, Qta. do Marquês, 2780-157 Oeiras, Portugal; ITQB - Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Quinta do Marquês, 2780-157 Oeiras, Portugal., Galinha CF; LAQV-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Portugal., Oehmen A; UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Portugal; School of Chemical Engineering, The University of Queensland, St Lucia, QLD 4072, Australia., Carvalho G; UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Portugal; Advanced Water Management Centre (AWMC), The University of Queensland, St Lucia, QLD 4072, Australia. Electronic address: g.carvalho@awmc.uq.edu.au.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2019 Feb 15; Vol. 651 (Pt 2), pp. 2460-2472. Date of Electronic Publication: 2018 Oct 11.
DOI: 10.1016/j.scitotenv.2018.10.132
Abstrakt: Few attempts have been made in previous studies to link the microbial community structure and function with nitrous oxide (N 2 O) emissions at full-scale wastewater treatment plants (WWTPs). In this work, high-throughput sequencing and reverse transcriptase-qPCR (RT-qPCR) was applied to activated sludge samples from three WWTPs for two seasonal periods (winter and summer) and linked with the N 2 O emissions and wastewater characteristics. The total N 2 O emissions ranged from 7.2 to 937.0 g N-N 2 O/day, which corresponds to an emission factor of 0.001 to 0.280% of the influent NH 4 -N being emitted as N 2 O. Those emissions were related to the abundance of Nitrotoga, Candidatus Microthrix and Rhodobacter genera, which were favored by higher dissolved oxygen (DO) and nitrate (NO 3 - ) concentrations in the activated sludge tanks. Furthermore, a relationship between the nirK gene expression and N 2 O emissions was verified. Detected N 2 O emission peaks were associated with different process events, related to aeration transition periods, that occurred during the regular operation of the plants, which could be potentially associated to increased emissions of the WWTP. The design of mitigation strategies, such as optimizing the aeration regime, is therefore important to avoid process events that lead to those N 2 O emissions peaks. Furthermore, this study also demonstrates the importance of assessing the gene expression of nosZ clade II, since its high abundance in WWTPs could be an important key to reduce the N 2 O emissions.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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