Novel Ca 2+ -independent C-type lectin involved in immune defense of the razor clam Sinonovacula constricta.

Autor: Shi Y; School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China., Zhao X; School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China., Wang Z; School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China., Shao Y; School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China., Zhang W; School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China., Bao Y; Zhejiang Key Laboratory of Aquatic Germplasm Resources, Zhejiang Wanli University, Ningbo, 315100, PR China. Electronic address: bobbao2001@gmail.com., Li C; School of Marine Sciences, Ningbo University, Ningbo, 315211, PR China. Electronic address: lichenghua@nbu.edu.cn.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2019 Jan; Vol. 84, pp. 502-508. Date of Electronic Publication: 2018 Oct 15.
DOI: 10.1016/j.fsi.2018.10.036
Abstrakt: C-type lectins (CTLs) are important pattern recognition molecules that participate in bacterial binding and agglutination by specific recognition of carbohydrates from pathogens. In this study, a full-length cDNA of CTL was cloned from Sinonovacula constricta (designated ScCTL-2). ScCTL-2 has a length of 981 bp, a 5'-untranslated region (UTR) of 47 bp, a short 3'-UTR of 37 bp, and an open reading frame (ORF) of 894 bp, which encodes a polypeptide of 298 amino acid residues. The deduced amino acid of ScCTL-2 possesses a conserved carbohydrate-recognition domain (CRD) similar to that of C 31 -E 171 . Spatial distribution analysis demonstrated that ScCTL-2 was constitutively expressed in all tested tissues, with dominant expression in foot and siphon and weak expression in hepatopancreas. The mRNA expression level of ScCTL-2 in gills and hepatopancreas was significantly upregulated at 6 and 12 h after challenge with the pathogen Vibrio parahaemolyticus. The recombinant ScCTL-2 showed specific binding and agglutinate capacities to all examined Gram-negative bacterial species, namely, Escherichia coli, Vibro anguillarum, and V. parahaemolyticus in a Ca 2+ -independent manner. However, these binding activities were not detected in Gram-positive Micrococcus luteus. Our results indicated that ScCTL-2 could be a novel pattern recognition receptor that can specifically recognize Gram-negative microorganisms in the innate immunity of S. constricta.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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