Mapping Molecular Datasets Back to the Brain Regions They are Extracted from: Remembering the Native Countries of Hypothalamic Expatriates and Refugees.

Autor:	Khan AM; UTEP Systems Neuroscience Laboratory, University of Texas at El Paso, El Paso, TX, USA. amkhan2@utep.edu.; Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA. amkhan2@utep.edu.; Border Biomedical Research Center, University of Texas at El Paso, El Paso, TX, USA. amkhan2@utep.edu., Grant AH; UTEP Systems Neuroscience Laboratory, University of Texas at El Paso, El Paso, TX, USA.; Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA.; Graduate Program in Pathobiology, University of Texas at El Paso, El Paso, TX, USA., Martinez A; UTEP Systems Neuroscience Laboratory, University of Texas at El Paso, El Paso, TX, USA.; Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA.; Graduate Program in Pathobiology, University of Texas at El Paso, El Paso, TX, USA., Burns GAPC; Information Sciences Institute, Viterbi School of Engineering, University of Southern California, Marina del Rey, CA, USA., Thatcher BS; VA Puget Sound Health Care System, Office of Research and Development Medical Research Service, Department of Veterans Affairs Medical Center, Seattle, WA, USA., Anekonda VT; VA Puget Sound Health Care System, Office of Research and Development Medical Research Service, Department of Veterans Affairs Medical Center, Seattle, WA, USA., Thompson BW; VA Puget Sound Health Care System, Office of Research and Development Medical Research Service, Department of Veterans Affairs Medical Center, Seattle, WA, USA., Roberts ZS; VA Puget Sound Health Care System, Office of Research and Development Medical Research Service, Department of Veterans Affairs Medical Center, Seattle, WA, USA., Moralejo DH; Division of Neonatology, Department of Pediatrics, University of Washington School of Medicine, Seattle, WA, USA., Blevins JE; VA Puget Sound Health Care System, Office of Research and Development Medical Research Service, Department of Veterans Affairs Medical Center, Seattle, WA, USA.; Division of Metabolism, Endocrinology, and Nutrition, Department of Medicine, University of Washington School of Medicine, Seattle, WA, USA.
Jazyk:	angličtina
Zdroj:	Advances in neurobiology [Adv Neurobiol] 2018; Vol. 21, pp. 101-193.
DOI:	10.1007/978-3-319-94593-4_6
Abstrakt:	This article focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from brain tissue to the original locations within the brain that they are derived from using digital atlas mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we highlight studies that have mined biochemical and molecular information from the hypothalamus and then lay out a strategy for how these data can be linked spatially to the mapped locations in a canonical brain atlas where the data come from, thereby allowing researchers to integrate these data with other datasets across multiple scales. A key methodology that enables atlas-based mapping of extracted datasets-laser-capture microdissection-is discussed in detail, with a view of how this technology is a bridge between systems biology and systems neuroscience.
Databáze:	MEDLINE
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