| Autor: |
Tasnádi G; Austrian Centre of Industrial Biotechnology, c/o.; Department of Chemistry, Organic & Bioorganic Chemistry University of Graz Heinrichstrasse 28 8010 Graz Austria., Jud W; Department of Chemistry, Organic & Bioorganic Chemistry University of Graz Heinrichstrasse 28 8010 Graz Austria., Hall M; Department of Chemistry, Organic & Bioorganic Chemistry University of Graz Heinrichstrasse 28 8010 Graz Austria., Baldenius K; White Biotechnology Research Biocatalysis BASF SE Carl-Bosch-Strasse 38 67056 Ludwigshafen Germany., Ditrich K; White Biotechnology Research Biocatalysis BASF SE Carl-Bosch-Strasse 38 67056 Ludwigshafen Germany., Faber K; Department of Chemistry, Organic & Bioorganic Chemistry University of Graz Heinrichstrasse 28 8010 Graz Austria. |
| Jazyk: |
angličtina |
| Zdroj: |
Advanced synthesis & catalysis [Adv Synth Catal] 2018 Jun 15; Vol. 360 (12), pp. 2394-2401. Date of Electronic Publication: 2018 May 02. |
| DOI: |
10.1002/adsc.201800306 |
| Abstrakt: |
Undesired product hydrolysis along with large amounts of waste in form of inorganic monophosphate by-product are the main obstacles associated with the use of pyrophosphate in the phosphatase-catalyzed synthesis of phosphate monoesters on large scale. In order to overcome both limitations, we screened a broad range of natural and synthetic organic phosphate donors with several enzymes on a broad variety of hydroxyl-compounds. Among them, acetyl phosphate delivered stable product levels and high phospho-transfer efficiency at the lower functional pH-limit, which translated into excellent productivity. The protocol is generally applicable to acid phosphatases and compatible with a range of diverse substrates. Preparative-scale transformations using acetyl phosphate synthesized from cheap starting materials yielded multiple grams of various sugar phosphates with up to 433 g L -1 h -1 space-time yield and 75% reduction of barium phosphate waste. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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