The effects of stress, cortisol administration and cortisol inhibition on black sea bass (Centropristis striata) sex differentiation.

Autor: Miller KA; Department of Biological Sciences, University of New Hampshire, Durham, NH 03824, USA., Kenter LW; Department of Biological Sciences, University of New Hampshire, Durham, NH 03824, USA., Breton TS; Division of Natural Sciences, University of Maine at Farmington, 173 High Street, Farmington, ME 04938, USA., Berlinsky DL; Department of Agriculture, Nutrition and Food Systems, University of New Hampshire, 177 Spaulding Hall, Durham, NH 03824, USA. Electronic address: David.berlinsky@unh.edu.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2019 Jan; Vol. 227, pp. 154-160. Date of Electronic Publication: 2018 Oct 13.
DOI: 10.1016/j.cbpa.2018.10.009
Abstrakt: Sex differentiation in many lower vertebrates (e.g. reptiles, amphibians, and fishes) can be influenced by environmental factors experienced during sensitive developmental periods. Environmental stressors, acting through cortisol, masculinize some teleost fishes during development by limiting gonadal cytochrome P450 aromatase (cyp19a1a), the enzyme that irreversibly converts testosterone to 17β-estradiol. In this study, we examined the influence of cortisol, cortisol inhibitors and a repeated, acute stressor (net-chasing) on sex differentiation in black sea bass (BSB; Centropristis striata), a protogynous hermaphroditic teleost. Wild-caught, sexually-undifferentiated, BSB juveniles (~90 mm) were collected from Rhode Island waters, raised in recirculating systems and fed diets supplemented with cortisol, a cortisol receptor antagonist (mifepristone), a cortisol synthesis inhibitor (metyrapone), or net-chased twice a week for two min until gonads were differentiated (77-89 days). Long term cortisol administration partially masculinized all female fish, but repeated net-chasing did not alter sex differentiation relative to the control group. Blocking cortisol receptor binding delayed sex differentiation in some individuals, but overall led to increased masculinization compared to control fish. The proportion of treatment fish that developed as males suggests a functionally, diandric protogynous reproductive strategy in this species. We also identified a glucocorticoid response element in the gonadal aromatase (cyp19a1a) promoter, indicating a possible relationship between cortisol and cyp19a1a gene expression.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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