OXA-48-Like-Producing Klebsiella pneumoniae in Southern Spain in 2014-2015.

Autor: Machuca J; Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Seville, Spain.; Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena, CSIC, Universidad de Sevilla, Seville, Spain.; Red Española de Investigación en Patología Infecciosa, Instituto de Salud Carlos III, Madrid, Spain., López-Cerero L; Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Seville, Spain llopez@us.es.; Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena, CSIC, Universidad de Sevilla, Seville, Spain.; Red Española de Investigación en Patología Infecciosa, Instituto de Salud Carlos III, Madrid, Spain.; Departamento de Microbiología, Universidad de Sevilla, Seville, Spain., Fernández-Cuenca F; Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Seville, Spain.; Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena, CSIC, Universidad de Sevilla, Seville, Spain.; Red Española de Investigación en Patología Infecciosa, Instituto de Salud Carlos III, Madrid, Spain., Mora-Navas L; Hospital Clínico Universitario Virgen de la Victoria, Malaga, Spain., Mediavilla-Gradolph C; Hospital Regional Universitario Carlos Haya Malaga, Malaga, Spain., López-Rodríguez I; Hospital Costa del Sol, Marbella, Spain., Pascual Á; Unidad de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Seville, Spain.; Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena, CSIC, Universidad de Sevilla, Seville, Spain.; Red Española de Investigación en Patología Infecciosa, Instituto de Salud Carlos III, Madrid, Spain.; Departamento de Microbiología, Universidad de Sevilla, Seville, Spain.
Jazyk: angličtina
Zdroj: Antimicrobial agents and chemotherapy [Antimicrob Agents Chemother] 2018 Dec 21; Vol. 63 (1). Date of Electronic Publication: 2018 Dec 21 (Print Publication: 2019).
DOI: 10.1128/AAC.01396-18
Abstrakt: The aim of this study was to characterize the population structure of 56 OXA-48-like-producing Klebsiella pneumoniae isolates, as well as extended-spectrum β-lactamase (ESBL) and carbapenemase genes, recovered in 2014 and 2015 from 16 hospitals in southern Spain. XbaI pulsed-field gel electrophoresis and multilocus sequence typing were performed to assess clonal relatedness. Representative isolates belonging to OXA-48-like-producing and CTX-M-15-coproducing pulsotypes were selected for characterization of bla OXA-48-like - and bla CTX-M-15 -carrying plasmids by PCR-based replicon typing, IncF subtyping, whole-genome sequencing analysis, and typing of Tn 1999 structures. Forty-three OXA-48-producing isolates (77%) were recovered from clinical samples and 13 from rectal swabs. All isolates showed ertapenem MIC values of ≥1 mg/liter, although 70% remained susceptible to imipenem and meropenem. Forty-nine isolates (88%) produced OXA-48, 5 produced OXA-245, and 2 produced OXA-181. Twenty-eight different pulsotypes (5 detected in more than 1 hospital) and 16 sequence types (STs) were found. The most prevalent clones were ST15 (29 isolates [52%]) and ST11 (7 isolates [13%]). Forty-five (80%) isolates were also bla CTX-M-15 carriers. The bla CTX-M-15 gene was mostly (82%) located on IncR plasmids, although ST15 and ST11 isolates also carried this gene on IncF plasmids. The composite transposon variant Tn 1999.2 -like was the most frequent. Among ST15 and ST11 isolates, different transposon variants were observed. The bla OXA-48 gene was mainly located on IncL plasmids, although IncM plasmids were also observed. The spread of OXA-48-like-producing K. pneumoniae in southern Spain is mainly due to ST15 and ST11 clones. Variation within clonal lineages could indicate different acquisition events for both ESBL and carbapenemase traits.
(Copyright © 2018 American Society for Microbiology.)
Databáze: MEDLINE
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