| Autor: |
Lin CZ; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Guangzhou 510405, China. linchaozhan@gzucm.edu.cn., Zhang RJ; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Guangzhou 510405, China. zhang_runjing@sina.com., Yao YF; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Guangzhou 510405, China. yao_yufeng@sina.com., Huang XD; Research & Development Institute, Guangzhou Wanglaoji Pharmaceutical Company Limited, No. 831 Guanghuaer Road, Guangzhou 510450, China. xiaodanhuang@126.com., Zheng RB; Research & Development Institute, Guangzhou Wanglaoji Pharmaceutical Company Limited, No. 831 Guanghuaer Road, Guangzhou 510450, China. zrbb0819@sina.com., Wu BJ; Research & Development Institute, Guangzhou Wanglaoji Pharmaceutical Company Limited, No. 831 Guanghuaer Road, Guangzhou 510450, China. wu_bojian@sina.com., Zhu CC; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Guangzhou 510405, China. zhucc@gzucm.edu.cn. |
| Abstrakt: |
Quality control of Chinese herbal tea remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling and famous brand of beverage in China, Wanglaoji Herbal Tea (WLJHT), via a full component quantitative analysis. In this paper, a total of thirty-two representative constituents were identified or tentatively characterized using ultra-high performance liquid chromatography coupled with quadrupole tandem time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Moreover, the quantitative analyses of fourteen constituents were performed by high performance liquid chromatography with a triple quadruple tandem mass spectrometry (HPLC-MS/MS) method and saccharide compositions of WLJHT were also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on a Hilic column, separately. Using multiple chromatographic techniques presented a good precision, sensitivity, repeatability and stability, and was successfully applied to analyze 16 batches of WLJHT samples. Therefore, it would be a reliable and useful approach for the quality control of WLJHT. |
