The Majority of CD45 -  Ter119 -  CD31 - Bone Marrow Cell Fraction Is of Hematopoietic Origin and Contains Erythroid and Lymphoid Progenitors.

Autor:	Boulais PE; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, New York, 10461, USA., Mizoguchi T; Institute for Oral Science, Matsumoto Dental University, Nagano, 399-0781 Japan., Zimmerman S; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, New York, 10461, USA; Department of Systems and Computational Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA., Nakahara F; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, New York, 10461, USA., Vivié J; Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Cancer Genomics Netherlands, 3584 CT Utrecht, the Netherlands., Mar JC; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, New York, 10461, USA; Department of Systems and Computational Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Department of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Australian Institute for Bioengineering and Nanotechnology, University of Queensland, Brisbane QLD 4072, Australia., van Oudenaarden A; Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Cancer Genomics Netherlands, 3584 CT Utrecht, the Netherlands., Frenette PS; Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA; Department of Medicine, Albert Einstein College of Medicine, Bronx, New York, 10461, USA; The Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, Bronx, New York, 10461, USA. Electronic address: paul.frenette@einstein.yu.edu.
Jazyk:	angličtina
Zdroj:	Immunity [Immunity] 2018 Oct 16; Vol. 49 (4), pp. 627-639.e6. Date of Electronic Publication: 2018 Oct 09.
DOI:	10.1016/j.immuni.2018.08.019
Abstrakt:	The non-hematopoietic cell fraction of the bone marrow (BM) is classically identified as CD45 - Ter119 - CD31 - (herein referred to as triple-negative cells or TNCs). Although TNCs are believed to contain heterogeneous stromal cell populations, they remain poorly defined. Here we showed that the vast majority of TNCs (∼85%) have a hematopoietic rather than mesenchymal origin. Single cell RNA-sequencing revealed erythroid and lymphoid progenitor signatures among CD51 - TNCs. Ly6D + CD44 + CD51 - TNCs phenotypically and functionally resembled CD45 + pro-B lymphoid cells, whereas Ly6D - CD44 + CD51 - TNCs were enriched in previously unappreciated stromal-dependent erythroid progenitors hierarchically situated between preCFU-E and proerythroblasts. Upon adoptive transfer, CD44 + CD51 - TNCs contributed to repopulate the B-lymphoid and erythroid compartments. CD44 + CD51 - TNCs also expanded during phenylhydrazine-induced acute hemolysis or in a model of sickle cell anemia. These findings thus uncover physiologically relevant new classes of stromal-associated functional CD45 - hematopoietic progenitors. (Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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