Critical Role for a Subset of Intestinal Macrophages in Shaping Gut Microbiota in Adult Zebrafish.
| Autor: | Earley AM; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Graves CL; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA., Shiau CE; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. Electronic address: shiauce@unc.edu. |
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| Jazyk: | angličtina |
| Zdroj: | Cell reports [Cell Rep] 2018 Oct 09; Vol. 25 (2), pp. 424-436. |
| DOI: | 10.1016/j.celrep.2018.09.025 |
| Abstrakt: | The gut microbiota is strongly influenced by environmental factors, although host contribution is far less understood. We leveraged macrophage-deficient interferon regulatory factor irf8 zebrafish mutants to investigate the role of macrophages in this process. In conventionally raised adult irf8-deficient mutants, we found a significant loss of intestinal macrophages associated with a strikingly altered gut microbiota when compared to co-housed siblings. The destabilization of the gut commensal microbiota was associated with a severe reduction in complement C1q genes and outgrowth of a rare bacterial species. Consistent with a critical function of irf8 in adult intestinal macrophages, irf8 is abundantly expressed in these cells normally, and restoring macrophage irf8 expression in irf8 mutants was sufficient to recover commensal microbes and C1q genes expression. This study reports an important subpopulation of intestinal macrophages that requires irf8 to establish in the gut, ensure normal colonization of gut microbes, and prevent immune dysregulation. (Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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