| Autor: |
Enriquez JS; Department of Chemistry, The University of Texas at Austin, 105 E. 24th St Stop A5300, Austin, Texas 78712, USA. emilyque@cm.utexas.edu., Yu M, Bouley BS, Xie D, Que EL |
| Jazyk: |
angličtina |
| Zdroj: |
Dalton transactions (Cambridge, England : 2003) [Dalton Trans] 2018 Oct 30; Vol. 47 (42), pp. 15024-15030. |
| DOI: |
10.1039/c8dt03780a |
| Abstrakt: |
Cysteine plays an essential role in maintaining cellular redox homeostasis and perturbations in cysteine concentration are associated with cardiovascular disease, liver disease, and cancer. 19F MRI is a promising modality for detecting cysteine in biology due to its high tissue penetration and negligible biological background signal. Herein we report fluorinated macrocyclic copper complexes that display a 19F NMR/MRI turn-on response following reduction of the Cu(ii) complexes by cysteine. The reactivity with cysteine was studied by monitoring the appearance of a robust diamagnetic 19F signal following addition of cysteine in conjunction with UV-vis and EPR spectroscopies. Importantly, complexes with -CH2CF3 tags display good water solubility. Studies with this complex in HeLa cells demonstrate the applicability of these probes to detect cysteine in complex biological environments. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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