Quantitative mass spectrometry to identify protein markers for diagnosis of malignant pleural mesothelioma.

Autor: White R; Department of Anatomical Pathology, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia., Pulford E; Department of Anatomical Pathology, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia., Elliot DJ; Department of Clinical Pharmacology, Flinders Medical Centre, Bedford Park, SA 5042, Australia., Thurgood LA; Molecular Medicine and Pathology, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia. Electronic address: lauren.thurgood@flinders.edu.au., Klebe S; Department of Anatomical Pathology, College of Medicine and Public Health, Flinders University, Bedford Park, SA 5042, Australia; Department of Surgical Pathology, SA Pathology, Flinders Medical Centre, Bedford Park, SA 5042, Australia.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2019 Feb 10; Vol. 192, pp. 374-382. Date of Electronic Publication: 2018 Oct 06.
DOI: 10.1016/j.jprot.2018.09.018
Abstrakt: Malignant pleural mesothelioma (MPM) is a devastating malignancy with a prognosis of <12 months. Even with bans on the use of asbestos in most Western countries, the incidence is still increasing due to the long latency periods between exposure and development of the disease. Diagnosis is often delayed due to invasive biopsies and lack of distinguishable markers. Patients frequently present with pleural effusions months to years before a radiologically detectable mass appears. This study aimed to investigate the proteome of pleural effusions taken from patients with MPM, adenocarcinoma and benign conditions in an attempt to identify a biomarker for early diagnosis. We identified several proteins that may be possible targets and warrant further investigation. Due to the predominance of up regulated proteins involved in VEGF signalling in MPM, we analysed VEGFA levels in effusions and found a strong correlation between VEGFA levels and survival in MPM.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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