Sepsis is associated with reduced spontaneous neutrophil migration velocity in human adults.

Autor: Raymond SL; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Hawkins RB; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Stortz JA; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Murphy TJ; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Ungaro R; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Dirain ML; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Nacionales DC; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Hollen MK; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Rincon JC; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Larson SD; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Brakenridge SC; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Moore FA; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Irimia D; Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, United States of America., Efron PA; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America., Moldawer LL; Department of Surgery, University of Florida College of Medicine, Gainesville, Florida, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Oct 09; Vol. 13 (10), pp. e0205327. Date of Electronic Publication: 2018 Oct 09 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0205327
Abstrakt: Sepsis is a common and deadly complication among trauma and surgical patients. Neutrophils must mobilize to the site of infection to initiate an immediate immune response. To quantify the velocity of spontaneous migrating blood neutrophils, we utilized novel microfluidic approaches on whole blood samples from septic and healthy individuals. A prospective study at a level 1 trauma and tertiary care center was performed with peripheral blood samples collected at <12 hours, 4 days, and/or 14 days relative to study initiation. Blood samples were also collected from healthy subjects. Ex vivo spontaneous neutrophil migration was measured on 2 μl of whole blood using microfluidic devices and time-lapse imaging. For each sample, individual neutrophils were tracked to calculate mean instantaneous velocity. Forty blood samples were collected from 33 patients with sepsis, and 15 blood samples were collected from age- and gender-matched healthy, control subjects. Average age was 61 years for septic patients with a male predominance (67%). Overall, average spontaneous neutrophil migration velocity in septic samples was 16.9 μm/min, significantly lower than controls samples at 21.1 μm/min (p = 0.0135). Neutrophil velocity was reduced the greatest at <12 hours after sepsis (14.5 μm/min). Regression analysis demonstrated a significant, positive correlation between neutrophil velocity and days after sepsis (p = 0.0059). There was no significant association between neutrophil velocity and age, gender, APACHE II score, SOFA score, sepsis severity, total white blood cell count, or percentage of neutrophils. Circulating levels of the cytokines IL-6, IL-8, IL-10, MCP-1, IP-10, and TNF were additionally measured using bead-based multiplex assay and found to peak at <12 hours and be significantly increased in patients with sepsis at all three time points (<12 hours, 4 days, and 14 days after sepsis) compared to healthy subjects. In conclusion, these findings may demonstrate an impaired ability of neutrophils to respond to sites of infection during the proinflammatory phase of sepsis.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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