| Autor: |
Tiwari DP; Food Science and Technology, Tata Chemicals Limited-Innovation Centre, Survey No. 315, Hissa No. 1-14, Ambedveth (V), Paud Road, Mulshi, Pune-412111, Maharashtra, India.; Department of Biotechnology, Manipal Institute of Technology-Manipal Academy of Higher Education, Manipal-576104, Karnataka, India., Chatterjee PM; Food Science and Technology, Tata Chemicals Limited-Innovation Centre, Survey No. 315, Hissa No. 1-14, Ambedveth (V), Paud Road, Mulshi, Pune-412111, Maharashtra, India.; Department of Biotechnology, Manipal Institute of Technology-Manipal Academy of Higher Education, Manipal-576104, Karnataka, India., Rotti H; Genotypic Technology [P] Ltd, #259, Apurva 4th Cross, 80 Feet Road, R.M.V. 2nd Stage, Bangaluru-560094, Karnataka, India., Chand B; Genotypic Technology [P] Ltd, #259, Apurva 4th Cross, 80 Feet Road, R.M.V. 2nd Stage, Bangaluru-560094, Karnataka, India., Raval R; Department of Biotechnology, Manipal Institute of Technology-Manipal Academy of Higher Education, Manipal-576104, Karnataka, India., Dubey AK; Food Science and Technology, Tata Chemicals Limited-Innovation Centre, Survey No. 315, Hissa No. 1-14, Ambedveth (V), Paud Road, Mulshi, Pune-412111, Maharashtra, India.; Genotypic Technology [P] Ltd, #259, Apurva 4th Cross, 80 Feet Road, R.M.V. 2nd Stage, Bangaluru-560094, Karnataka, India. |
| Abstrakt: |
Poly-γ-glutamic acid (PGA) is biosynthesized by various Bacillus species through PGA synthetase, encoded by the PGA operon comprised of the ywsC and ywtABC genes. Due to the minimal available knowledge, understanding the expression pattern of PGA operon genes is pivotal. In this study, the effect of glucose and glutamic acid on the global gene expression profile of Bacillus subtilis Natto3 was investigated using high throughput microarray, with an emphasis on the PGA operon and genes influencing PGA production. Two treatment groups (set1-in the presence of glutamic acid and set2-in the presence of glutamic acid + glucose) were analyzed against the control (in the presence of glucose). In the microarray, both the groups showed a trend of up-regulation for ywsC and ywtA genes (log2 fold change of 0.55, P = 0.0194, 0.92, P = 0.0069 in set1 and 0.78, P = 0.0023, 0.59, P = 0.0172 in set2, respectively) and down-regulation of ywtB and ywtC genes (log2 fold change of -1.83, P = 0.0001, -1.42, P = 0.0017 in set1 and -1.52, P = 0.0012, -0.55, P = 0.1112 in set2, respectively), supporting the indispensability of the ywsC and ywtA genes in PGA production. Interestingly, the ywtB and ywtC genes, belonging to the same operon, were down-regulated in both the conditions (set1 and set2). To the best of our knowledge, this expression pattern of PGA operon genes is a unique observation. |
