Heterochromatin restricts the mobility of nuclear bodies.

Autor: Arifulin EA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia., Sorokin DV; Laboratory of Mathematical Methods of Image Processing, Faculty of Computational Mathematics and Cybernetics, Lomonosov Moscow State University, 119991, Moscow, Russia., Tvorogova AV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia., Kurnaeva MA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia., Musinova YR; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia.; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Vavilov str. 26, 119334, Moscow, Russia., Zhironkina OA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia., Golyshev SA; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia., Abramchuk SS; Faculty of Chemistry, Lomonosov Moscow State University, 119991, Moscow, Russia., Vassetzky YS; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Vavilov str. 26, 119334, Moscow, Russia. vassetzky@igr.fr.; LIA 1066 LFR2O French-Russian Joint Cancer Research Laboratory, 94805, Villejuif, France. vassetzky@igr.fr.; UMR8126, CNRS, Institut de Cancérologie Gustave Roussy, Université Paris-Sud, 94805, Villejuif, France. vassetzky@igr.fr., Sheval EV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991, Moscow, Russia. sheval_e@belozersky.msu.ru.; LIA 1066 LFR2O French-Russian Joint Cancer Research Laboratory, 94805, Villejuif, France. sheval_e@belozersky.msu.ru.; Department of Cell Biology and Histology, Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow, Russia. sheval_e@belozersky.msu.ru.
Jazyk: angličtina
Zdroj: Chromosoma [Chromosoma] 2018 Dec; Vol. 127 (4), pp. 529-537. Date of Electronic Publication: 2018 Oct 05.
DOI: 10.1007/s00412-018-0683-8
Abstrakt: Nuclear bodies are relatively immobile organelles. Here, we investigated the mechanisms underlying their movement using experimentally induced interphase prenucleolar bodies (iPNBs). Most iPNBs demonstrated constrained diffusion, exhibiting infrequent fusions with other iPNBs and nucleoli. Fusion events were actin-independent and appeared to be the consequence of stochastic collisions between iPNBs. Most iPNBs were surrounded by condensed chromatin, while fusing iPNBs were usually found in a single heterochromatin-delimited compartment ("cage"). The experimentally induced over-condensation of chromatin significantly decreased the frequency of iPNB fusion. Thus, the data obtained indicate that the mobility of nuclear bodies is restricted by heterochromatin.
Databáze: MEDLINE
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