A novel, green, low-cost chitosan-starch hydrogel as potential delivery system for plant growth-promoting bacteria.

Autor: Perez JJ; Grupo de Aplicaciones de Materiales Biocompatibles, Departamento de Química, Facultad de Ingeniería, Universidad de Buenos Aires (UBA), C1063ACV, CABA, Argentina; Instituto de Tecnología en Polímeros y Nanotecnología (ITPN), Facultad de Ingeniería, Universidad de Buenos Aires (UBA)-CONICET, C1127AAQ, CABA, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1425FQB, Godoy Cruz 2290, CABA, Argentina., Francois NJ; Grupo de Aplicaciones de Materiales Biocompatibles, Departamento de Química, Facultad de Ingeniería, Universidad de Buenos Aires (UBA), C1063ACV, CABA, Argentina; Instituto de Tecnología en Polímeros y Nanotecnología (ITPN), Facultad de Ingeniería, Universidad de Buenos Aires (UBA)-CONICET, C1127AAQ, CABA, Argentina., Maroniche GA; Laboratorio Bioquímica Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, Km 73.5, Balcarce, B7620, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1425FQB, Godoy Cruz 2290, CABA, Argentina., Borrajo MP; Laboratorio Bioquímica Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, Km 73.5, Balcarce, B7620, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1425FQB, Godoy Cruz 2290, CABA, Argentina., Pereyra MA; Laboratorio Bioquímica Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, Km 73.5, Balcarce, B7620, Argentina., Creus CM; Laboratorio Bioquímica Vegetal y Microbiana, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, Ruta 226, Km 73.5, Balcarce, B7620, Argentina. Electronic address: ccreus@mdp.edu.ar.
Jazyk: angličtina
Zdroj: Carbohydrate polymers [Carbohydr Polym] 2018 Dec 15; Vol. 202, pp. 409-417. Date of Electronic Publication: 2018 Jul 30.
DOI: 10.1016/j.carbpol.2018.07.084
Abstrakt: The study examines the use of macrobeads for the controlled-release of bacteria. Macrobeads were prepared by an easy dripping-technique using 20/80 wt/wt chitosan-starch blends and sodium tripolyphosphate as cross-linking agent. The resulting polymeric matrix was examined by SEM, XRD, TGA, and solid-RMN. The swelling-equilibrium, thermal behaviour, crystallinity, and size of macrobeads were affected by the autoclave-sterilization. The diameter of the sterilized xerogel was c.a. 1.6 mm. The results suggested that ionotropic-gelation and neutralization were the mechanisms underlying hydrogel formation. Plant growth-promoting bacteria (PGPB) were loaded into macrobeads separately or co-inoculated. Bacteria loaded macrobeads were dried and stored. Bacteria survived at least 12 months in orders of 10 9 CFU of A. brasilense/g and 10 8 CFU of P. fluorescens/g. Bacterial release in sterile saline solution tended to a super Case-II transport mechanism. Polymeric-matrix release efficiently both PGPB in natural soils, which uncovers their potential for the formulation of novel and improved biofertilizers.
(Copyright © 2018. Published by Elsevier Ltd.)
Databáze: MEDLINE
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