Diagnosis of Avian Mycoplasmas: A Comparison between PCR and Culture Technique.

Autor:	Muhammad F; Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan.; Department of Microbiology, Faculty of Life Sciences & Informatics Balochistan University of IT, Engineering & Management Sciences (BUITEMS), Quetta Takkatu Campus, Airport road Quetta, Balochistan, Pakistan.; Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan., Hussain J; Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan., Fareed SK; Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan., Ahmad Khan T; Department of Physiology, Faculty of Science, University of Karachi, Karachi, Pakistan., Ahmad Khan S; Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan.; Department of Biosciences, Faculty of Science, Barrett Hodgson University, Karachi, Pakistan., Ahmad A; Department of Microbiology, Faculty of Science, University of Karachi, Karachi, Pakistan.; Department of Biosciences, Faculty of Science, Barrett Hodgson University, Karachi, Pakistan.
Jazyk:	angličtina
Zdroj:	Archives of Razi Institute [Arch Razi Inst] 2018 Sep; Vol. 73 (3), pp. 239-244. Date of Electronic Publication: 2018 Sep 01.
DOI:	10.22092/ari.2017.108217.1085
Abstrakt:	Mycoplasma gallisepticum and Mycoplasma synoviae are the causative agents of avian mycoplasmosis in commercial poultry. Among the available tools, polymerase chain reaction (PCR) and culture are confirmatory tools for the diagnosis of mycoplasmosis after the initial serological screening of suspected birds. Overall, 181 samples were analyzed, 152 (84%) and 103 (57%) of which were found positive by PCR and culture, respectively. Further, 54 (92%) broiler samples were found positive for general avian mycoplasma. Among the total positive samples, MS positivity was as high as 72 (47%) by PCR, while it was 45 (44%) by culture. MG positivity was 23% and 25% in PCR- and culture-positive samples. MG grows more easily compared to MS. The agreement value between the tests was 67%. Overall, flock wise prevalence was not much varied. The prevalence of mycoplasmosis was higher during winter. Our study confirmed that PCR is the most sensitive and reliable tool for the diagnosis of avian mycoplasmosis in field samples. (Copyright © 2018, Archives of Razi Institute. Published by Kowsar.)
Databáze:	MEDLINE
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