| Autor: |
Colello R; Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, UNCPBA 7000, Argentina. rocioc@vet.unicen.edu.ar.; Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA 7000, Argentina. rocioc@vet.unicen.edu.ar., Krüger A; Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, UNCPBA 7000, Argentina. akruger@vet.unicen.edu.ar.; Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA 7000, Argentina. akruger@vet.unicen.edu.ar., Conza JD; Laboratorio de Resistencia Microbiana, Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires 1426, Argentina. jdiconza@gmail.com., Rossen JWA; Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands. j.w.a.rossen@rug.nl (J.W.A.R.). j.w.a.rossen@rug.nl., Friedrich AW; Department of Medical Microbiology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands. j.w.a.rossen@rug.nl (J.W.A.R.). alex.friedrich@umcg.nl., Gutkind G; Laboratorio de Resistencia Microbiana, Cátedra de Microbiología, Facultad de Farmacia y Bioquímica, UBA, Buenos Aires 1426, Argentina. ggutkind@ffyb.uba.ar., Etcheverría AI; Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, UNCPBA 7000, Argentina. analiain@vet.unicen.edu.ar.; Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA 7000, Argentina. analiain@vet.unicen.edu.ar., Padola NL; Laboratorio de Inmunoquímica y Biotecnología, Facultad de Ciencias Veterinarias, UNCPBA 7000, Argentina. nlpadola@vet.unicen.edu.ar.; Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, CICPBA 7000, Argentina. nlpadola@vet.unicen.edu.ar. |
| Abstrakt: |
The aim of this study was to investigate the presence of class 1 integrons in a collection of Shiga toxin-producing Escherichia coli (STEC) from different origins and to characterize pheno- and genotypically the antimicrobial resistance associated to them. A collection of 649 isolates were screened for the class 1 integrase gene ( intI1 ) by Polymerase chain reaction The variable region of class 1 integrons was amplified and sequenced. Positive strains were evaluated for the presence of antimicrobial resistance genes with microarray and for antimicrobial susceptibility by the disk diffusion method. Seven out of 649 STEC strains some to serogroups, O26, O103 and O130 isolated from cattle, chicken burger, farm environment and pigs were identified as positive for intl1 . Different arrangements of gene cassettes were detected in the variable region of class 1 integron: dfrA16 , aadA23 and dfrA1-aadA1 . In almost all strains, phenotypic resistance to streptomycin, tetracycline, trimethoprim/sulfamethoxazole, and sulfisoxazole was observed. Microarray analyses showed that most of the isolates carried four or more antimicrobial resistance markers and STEC strains were categorized as Multridrug-resistant. Although antimicrobials are not usually used in the treatment of STEC infections, the presence of Multridrug-resistant in isolates collected from farm and food represents a risk for animal and human health. |
