Phytosynthesis of gold and silver nanoparticles enhance in vitro antioxidant and mitostimulatory activity of Aconitum toxicum Reichenb. rhizomes alcoholic extracts.

Autor: Sutan NA; University of Piteşti, Faculty of Science, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Arges, Romania., Manolescu DS; University of Piteşti, Faculty of Science, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Arges, Romania., Fierascu I; National Institute for Research & Development in Chemistry and Petrochemistry - ICECHIM Bucharest, 202 Spl. Independentei, 060021 Bucharest, Romania., Neblea AM; University of Piteşti, Faculty of Science, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Arges, Romania., Sutan C; University of Piteşti, Faculty of Science, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Arges, Romania., Ducu C; University of Pitesti, Regional Research & Development Center for Innovative Materials, Products and Processes from Automotive Industry, 11 Doaga Str., 110440 Pitesti, Arges, Romania., Soare LC; University of Piteşti, Faculty of Science, Physical Education and Informatics, Department of Natural Sciences, 1 Targu din Vale Str., 110040 Pitesti, Arges, Romania., Negrea D; University of Pitesti, Regional Research & Development Center for Innovative Materials, Products and Processes from Automotive Industry, 11 Doaga Str., 110440 Pitesti, Arges, Romania., Avramescu SM; University of Agronomic Sciences and Veterinary Medicine Bucharest, 59 Mărăşti Bvd, 011464 Bucharest, Romania., Fierascu RC; National Institute for Research & Development in Chemistry and Petrochemistry - ICECHIM Bucharest, 202 Spl. Independentei, 060021 Bucharest, Romania. Electronic address: radu_claudiu_fierascu@yahoo.com.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2018 Dec 01; Vol. 93, pp. 746-758. Date of Electronic Publication: 2018 Aug 22.
DOI: 10.1016/j.msec.2018.08.042
Abstrakt: Extracts obtained from different plant species proved to be a valuable tool in various biomedical applications. In the same time, the phytosynthesis of noble metal nanoparticles represents an already well-established route for obtaining nanoparticles with biological activity. The present paper studies the antioxidant activity and the cytogenetic effects of the alcoholic extracts from rhizomes of Aconitum toxicum Rchb., before and after the phytosynthesis of gold and silver nanoparticles, on the meristematic root cells of Allium cepa L., and on the general mitotic index and the progression rate through the mitotic phases, respectively, as well as on the genetic material organized in chromosomes. The extracts were characterized in terms of total polyphenolics content (1.49% and, respectively, 2.29%) and aconitine content (by HPLC - 4.891 mg/L and, respectively, 18.211 mg/L), while the phytosynthesis of metallic nanoparticles was monitored by UV-Vis spectrometry, X-ray diffraction, X-ray fluorescence and electron microscopy. Both the extracts and the obtained nanoparticles were evaluated for antioxidant potential (the antioxidant activity ranging between 78% and 84.32%) and cytogenetic effects. The obtained results prove the phytosynthesis of AgNPs and AuNPs with dimensions ranging from 9 nm to 15 nm for AuNPs, respectively from 53 nm to 67 nm for AgNPs. The extracts obtained from rhizomes of A. toxicum Rchb. induced mitotic stress, as well as a series of nuclear and mitotic aberrations. The biosynthesis of AgNPs and AuNPs intensified the antioxidant and mitostimulatory activity of the extracts.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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