Protective nature of low molecular weight chitosan in a chitosan-Amphotericin B nanocomplex - A physicochemical study.

Autor: Krishnan RA; Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, Maharashtra, India; Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, Maharashtra, India., Pant T; Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, Maharashtra, India., Sankaranarayan S; Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, Maharashtra, India., Stenberg J; Ridgeview Instruments AB, Vänge, Sweden., Jain R; Department of Chemical Engineering, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, Maharashtra, India. Electronic address: rd.jain@ictmumbai.edu.in., Dandekar P; Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, Nathalal Parekh Marg, Matunga, Mumbai 400019, Maharashtra, India. Electronic address: pd.jain@ictmumbai.edu.in.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2018 Dec 01; Vol. 93, pp. 472-482. Date of Electronic Publication: 2018 Aug 07.
DOI: 10.1016/j.msec.2018.08.016
Abstrakt: Chitosan, a biopolymer of immense potential, has been well-explored over the past decade in the biomedical field. Despite its numerous biological properties like anti-microbial, anti-inflammatory, anti-diabetic etc., limited studies have been conducted on its ability to protect therapeutic molecules in nano-formulations. Amphotericin B (AMP) is one such therapeutic molecule which requires protection as it possesses inherent limitations of poor water-solubility, susceptibility to oxidation- and/or light-induced degradation etc. Although AMP formulations have been quite successful in treating chronic fungal infections, their high cost, prolonged nature of therapy and instability over longer durations has necessitated the development of alternative carriers. In the present study, a stable nanoparticulate formulation was successfully prepared using low molecular weight chitosan and the anti-fungal agent AMP and this was found to offer protection to the labile AMP. The developed nanocomplexes could prevent degradation of AMP up to six months, in solution form, unlike the native drug which degraded in <24 h. Antifungal studies of the developed nanocomplexes revealed a surface charge-dependent antifungal activity. Furthermore, the nanocomplexes did not affect the viability of mammalian cells and showed excellent intracellular uptake and retention, and hence suggested potential of the nanocomplexes in alleviating systemic fungal infections.
(Copyright © 2018. Published by Elsevier B.V.)
Databáze: MEDLINE
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