| Autor: |
Oyelade AA; Department of Microbiology, University of Ibadan, Ibadan, Nigeria. oyeladebolade@gmail.com.; New Jersey Department of Environmental Protection, Leeds Point Office, Leeds Point, NJ, USA. oyeladebolade@gmail.com., Adelowo OO; Department of Microbiology, University of Ibadan, Ibadan, Nigeria., Fagade OE; Department of Microbiology, University of Ibadan, Ibadan, Nigeria. |
| Jazyk: |
angličtina |
| Zdroj: |
Environmental science and pollution research international [Environ Sci Pollut Res Int] 2018 Nov; Vol. 25 (33), pp. 33538-33547. Date of Electronic Publication: 2018 Sep 29. |
| DOI: |
10.1007/s11356-018-3306-2 |
| Abstrakt: |
Twenty-six strains of Vibrio parahaemolyticus and 14 strains of V. vulnificus isolated from selected beaches in Lagos State, Nigeria, were examined for virulence and antimicrobial resistance genes. The V. parahaemolyticus isolates were further serotyped and subjected to pulsed field gel electrophoresis (PFGE). Five strains of V. vulnificus and one of V. parahaemolyticus carried the New Delhi-metallo-beta-lactamase gene bla NDM-1 , seven strains carried bla TEM , and four strains of V. vulnificus and one of V. parahaemolyticus carried bla CMY . Real-time PCR assay for detection of virulence genes tdh and trh in the V. parahaemolyticus isolates showed that five isolates were positive for tdh, two for trh, and one isolate carried both genes. Ten V. parahaemolyticus serogroups and 23 pulsotypes were identified from 26 isolates based on O and K antigens typing and PFGE. Five of the isolates belong to the pandemic strains O1:Kut and O3:K6, and three belonged to the highly virulent O4:Kut serotype. Nineteen of the isolates showed distinct PFGE banding patterns. These results highlighted the importance of Nigerian recreational beaches as reservoirs of antimicrobial resistance genes of global public health interest, such as bla NDM-1 . |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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