Citrobacter freundii infection in silver catfish (Rhamdia quelen): Hematological and histological alterations.

Autor: Bandeira Junior G; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Dos Santos AC; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Souza CF; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Baldissera MD; Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Moreira KLDS; Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., da Veiga ML; Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., da Rocha MIUM; Department of Morphology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., de Vargas APC; Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., da Cunha MA; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil., Baldisserotto B; Department of Physiology and Pharmacology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: bbaldisserotto@hotmail.com.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2018 Dec; Vol. 125, pp. 276-280. Date of Electronic Publication: 2018 Sep 25.
DOI: 10.1016/j.micpath.2018.09.038
Abstrakt: Citrobacter freundii is a fish pathogen known for its ability to cause injury and high mortality. There have been no studies reporting the effect of this bacterium on hematological parameters and internal organ histology in silver catfish (Rhamdia quelen). Therefore, the aim of this study is to evaluate the hematological and histopathological effects of an experimentally induced C. freundii infection in silver catfish. Twenty fish were divided into healthy and infected groups. The fish of the infected group were inoculated intramuscularly with 100 μL of bacterial suspension (6.4 × 10 8  CFU mL -1 ), while healthy control animals received 100 μL of sterile saline. On day 18 post-infection, blood and tissues (cephalic kidneys, livers, and spleens) were collected for histological analysis. The infected animals presented high mortality, as well as hematological and histological changes. In relation to hematology, the infected fish presented aregenerative anemia, protein loss, leukopenia with neutropenia, lymphocytosis, and leukoblastosis. Regarding histology, there was liver degeneration, decrease in the amount of renal hematopoietic tissue, and the presence of melanomacrophage centers (MMCs) in the spleen and cephalic kidney of infected fish. In summary, these alterations may contribute to disease pathophysiology, contributing to high mortality of affected fish.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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