Expression of the O -Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa.

Autor: Nygaard MB; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. marie.berg.nygaard@regionh.dk.; The Fertility Clinic, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. marie.berg.nygaard@regionh.dk., Herlihy AS; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. menisca@gmail.com., Jeanneau C; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. charlotte.jeanneau@univ-amu.fr., Nielsen JE; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. JOHN.ERIK.NIELSEN@regionh.dk.; International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. JOHN.ERIK.NIELSEN@regionh.dk., Bennett EP; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. epb@sund.ku.dk., Jørgensen N; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. Niels.Joergensen@regionh.dk.; International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. Niels.Joergensen@regionh.dk., Clausen H; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. hclau@sund.ku.dk., Mandel U; Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine and Odontology, Faculty of health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. ulma@sund.ku.dk., Rajpert-De Meyts E; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. Ewa.Rajpert-De.Meyts@regionh.dk.; International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. Ewa.Rajpert-De.Meyts@regionh.dk., Almstrup K; Department of Growth and Reproduction, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. kristian.almstrup@regionh.dk.; International Center for Research and Research Training in Endocrine Disruption of Male Reproduction and Child Health (EDMaRC), Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen Ø, Denmark. kristian.almstrup@regionh.dk.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2018 Sep 27; Vol. 19 (10). Date of Electronic Publication: 2018 Sep 27.
DOI: 10.3390/ijms19102949
Abstrakt: We question whether the expression of GalNAc-T3, the only known O -GalNAc-transferase present in germ cells, is correlated with qualitative and functional parameters of spermatozoa. We investigated the expression of GalNAc-T3 in ejaculated spermatozoa with immunocytochemistry in swim-up purified and acrosome-reacted spermatozoa from quality-control semen donors and in semen samples from 206 randomly selected men representing a broad spectrum of semen quality. Using donor ejaculates and immunofluorescence detection we found that expression of GalNAc-T3 and the presence of the immature O -glycans Tn and T localized to the equatorial segment of spermatozoa. The proportion of GalNAc-T3-positive spermatozoa in the ejaculate increased after swim-up and appeared unaffected by induction of acrosomal exocytosis. The fraction of spermatozoa with equatorial expression of GalNAc-T3 correlated with classical semen parameters (concentration p = 9 × 10 -6 , morphology p = 7 × 10 -8 , and motility p = 1.8 × 10 -5 ) and was significantly lower in men with oligoteratoasthenozoospermia ( p = 0.0048). In conclusion, GalNAc-T3 was highly expressed by motile spermatozoa and the expression correlated positively with the classical semen parameters. Therefore, GalNAc-T3 expression seems related to the quality of the spermatozoa, and we propose that reduced expression of GalNAc-T3 may lead to impaired O -glycosylation of proteins and thereby abnormal maturation and reduced functionality of the spermatozoa.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje