The effect of 2.45 GHz non-ionizing radiation on the structure and ultrastructure of the testis in juvenile rats.

Autor:	Šimaiová V; Department of Anatomy, Histology and Physiology, Institute of Histology and Embryology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia. nika.simaiova@gmail.com., Almášiová V; Department of Anatomy, Histology and Physiology, Institute of Histology and Embryology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia., Holovská K; Department of Anatomy, Histology and Physiology, Institute of Histology and Embryology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia., Kisková T; Department of Animal Physiology, Institute of Biology and Ecology, Faculty of Sciences, Pavol Jozef Šafárik University in Košice, Slovakia., Horváthová F; Department of Anatomy, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Slovakia., Ševčíková Z; Department of Pathological Anatomy and Pathological Physiology, Institute of Pathological Anatomy, University of Veterinary Medicine and Pharmacy in Košice, Slovakia., Tóth Š; Department of Histology and Embryology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Slovakia., Raček A; Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia., Račeková E; Institute of Neurobiology of Biomedical Research Center of Slovak Academy of Sciences, Košice, Slovakia., Beňová K; Department of Biology and Genetics, Institute of Biology, Zoology and Radiobiology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia., Dvořák P; Department of Gastronomy, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic., Cigánková V; Department of Anatomy, Histology and Physiology, Institute of Histology and Embryology, University of Veterinary Medicine and Pharmacy in Košice, Slovakia.
Jazyk:	angličtina
Zdroj:	Histology and histopathology [Histol Histopathol] 2019 Apr; Vol. 34 (4), pp. 391-403. Date of Electronic Publication: 2018 Sep 27.
DOI:	10.14670/HH-18-049
Abstrakt:	Background: Nowadays, mobile devices that emit non-ionizing electromagnetic radiation (EMR) are predominantly used by juveniles and pubescents. The aim of the present study was to evaluate the effect of whole body pulsed EMR on the juvenile Wistar albino rat testis at a frequency of 2.45 GHz and mean power density of 2.8 mW/cm². Methods: The investigated animals (n=24) were divided into two control and two EMR groups (5 and 6 week old rats; 6 rats per group). Both EMR groups were irradiated continually for 3 weeks (2h/day) from postnatal days 14 and 21, respectively. Results: EMR caused an irregular shape of seminiferous tubules with desquamated immature germ cells in the lumen, a large number of empty spaces along the seminiferous epithelium and dilated and congested blood vessels in the interstitial tissue of the testis. The cytoplasm of Sertoli cells showed strong vacuolization and damaged organelles, with the cytoplasm full of different heterophagic and lipid vacuoles or the cytoplasm of spermatocytes with swollen mitochondria in both irradiated groups. A significant increase in the total tubular area of seminiferous tubules was observed in both EMR groups compared with controls (P<0.001). A significant increase in the TUNEL-positive apoptotic nuclei (P<0.01) was accompanied by a significant rise in both Cu-Zn-SOD (P<0.01) and Mn-SOD (P<0.001) positive cells in the 6 week old experimental rats compared to control animals. Conclusion: Our results confirmed a harmful effect of non-ionizing radiation on the structure and ultrastructure of the juvenile rat testis.
Databáze:	MEDLINE
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