Dextran adsorption onto red blood cells revisited: single cell quantification by laser tweezers combined with microfluidics.

Autor:	Lee K; Experimental Physics, Saarland University, Saarbrücken, D-66041, Germany.; Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russia.; Curremtly with Ulsan National Institute of Science and Technology, Institute for Basic Science, Center for Soft and Living Matter, Ulsan, 44919, South Korea.; Co-first authors with equal contribution., Shirshin E; Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russia.; Co-first authors with equal contribution., Rovnyagina N; Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russia., Yaya F; Experimental Physics, Saarland University, Saarbrücken, D-66041, Germany.; Laboratoire Interdisciplinaire de Physique, UMR 5588 CNRS and University Grenoble-Alpes, Saint Martin d'Hères Cedex, B.P. 87, 38402, France., Boujja Z; Experimental Physics, Saarland University, Saarbrücken, D-66041, Germany.; LaMCScI, University Mohamed V, Faculty of Sciences, Rabat, Morocco., Priezzhev A; Faculty of Physics, Lomonosov Moscow State University, Moscow, 119991, Russia.; International Laser Center, Lomonosov Moscow State University, Moscow, 119991, Russia., Wagner C; Experimental Physics, Saarland University, Saarbrücken, D-66041, Germany.; Physics and Materials Science Research Unit, University of Luxembourg, L-1511, Luxembourg.
Jazyk:	angličtina
Zdroj:	Biomedical optics express [Biomed Opt Express] 2018 May 22; Vol. 9 (6), pp. 2755-2764. Date of Electronic Publication: 2018 May 22 (Print Publication: 2018).
DOI:	10.1364/BOE.9.002755
Abstrakt:	The aggregation of red blood cells (RBC) is of importance for hemorheology, while its mechanism remains debatable. The key question is the role of the adsorption of macromolecules on RBC membranes, which may act as "bridges" between cells. It is especially important that dextran is considered to induce "bridge"-less aggregation due to the depletion forces. We revisit the dextran-RBC interaction on the single cell level using the laser tweezers combined with microfluidic technology and fluorescence microscopy. An immediate sorption of ~10 4 molecules of 70 kDa dextran per cell was observed. During the incubation of RBC with dextran, a gradual tenfold increase of adsorption was found, accompanied by a moderate change in the RBC deformability. The obtained data demonstrate that dextran sorption and incubation-induced changes of the membrane properties must be considered when studying RBC aggregation in vitro . Competing Interests: The authors declare that there are no conflicts of interest related to this article.
Databáze:	MEDLINE
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