Attenuation of Remifentanil-Induced Hyperalgesia by Betulinic Acid Associates with Inhibiting Oxidative Stress and Inflammation in Spinal Dorsal Horn.

Autor:	Lv CC; Department of Anesthesiology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China., Xia ML; Institute of Physiological Function, Medical College of Jiaxing University, Jiaxing, China., Shu SJ; Department of Obstetrics, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China., Chen F; Institute of Physiological Function, Medical College of Jiaxing University, Jiaxing, China., Jiang LS; Department of Anesthesiology and Pain Clinic, The First Affiliated Hospital of Jiaxing University, Jiaxing, China, jianglishan@mail.zjxu.edu.cn.
Jazyk:	angličtina
Zdroj:	Pharmacology [Pharmacology] 2018; Vol. 102 (5-6), pp. 300-306. Date of Electronic Publication: 2018 Sep 25.
DOI:	10.1159/000493144
Abstrakt:	Remifentanil-induced hyperalgesia (RIH) is known to be associated with oxidative stress and inflammation. Betulinic acid (BA) was reported to reduce visceral pain owing to its anti-oxidative and anti-inflammatory potential. Here, we -explored whether BA can attenuate RIH through inhibiting oxidative stress and inflammation in spinal dorsal horn. Sprague-Dawley rats were randomly divided into 4 groups: Control, Incision, RIH, and RIH pre-treated with BA. After pretreated with BA (25 mg/kg, i.g.) for 7 days, rats were subcutaneously infused with remifentanil (40 μg/kg) for 30 min during right plantar incision surgery to induce RIH. The paw withdrawal mechanical threshold (PWMT), paw withdrawal thermal latency (PWTL), spinal oxidative stress and inflammatory mediators were determined. Intraoperative remifentanil infusion induced postoperative hyperalgesia, as evidenced by the significant decrease in PWMT and PWTL (p < 0.01), and the significant increase in oxidative stress and inflammation evidenced by up-regulations of malondialdehyde, 3-nitrotyrosine, interleukin-1β and tumour necrosis factor-α (p < 0.01) in spinal dorsal horn and matrix metalloproteinase-9 (MMP-9) activity (p < 0.01) in dorsal root ganglion, as well as a decrease in manganese superoxide -dismutase activity (p < 0.01) compared with control and -incision groups. All these results mentioned above were markedly reversed by pre-treatment with BA (p < 0.01) compared with RIH group. These findings demonstrated that BA can effectively attenuate RIH, which associates with potentially inhibiting oxidative stress and subsequently down-regulating MMP-9-related pro-inflammatory cyokines in spinal dorsal horn. (© 2018 S. Karger AG, Basel.)
Databáze:	MEDLINE
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