Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences.

Autor: Wan WL; Department of Plant Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany., Zhang L; Department of Plant Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany., Pruitt R; Department of Plant Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany., Zaidem M; Department of Molecular Biology, Max-Planck-Institute for Developmental Biology, Max-Planck-Str. 5, D-72076, Tübingen, Germany.; Center for Genomics & Systems Biology, New York University, 12 Waverly Place, New York, NY, 10003, USA., Brugman R; Centre for Organismal Studies & Excellence Cluster Cell Networks, Heidelberg University, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany., Ma X; Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX, 77843, USA., Krol E; Plant Physiology and Biophysics, Julius Maximilians University Würzburg, Julius-von-Sachs-Platz 2, 97082, Würzburg, Germany.; Department of Biophysics, Institute of Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033, Lublin, Poland., Perraki A; The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.; Department of Plant Sciences, University of Cambridge, Cambridge, CB2 3EA, UK., Kilian J; Analytics Unit, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany., Grossmann G; Centre for Organismal Studies & Excellence Cluster Cell Networks, Heidelberg University, Im Neuenheimer Feld 230, 69120, Heidelberg, Germany., Stahl M; Analytics Unit, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany., Shan L; Institute for Plant Genomics & Biotechnology, Texas A&M University, College Station, TX, 77843, USA., Zipfel C; The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK., van Kan JAL; Laboratory of Phytopathology, Wageningen University, 6708 PB, Wageningen, the Netherlands., Hedrich R; Plant Physiology and Biophysics, Julius Maximilians University Würzburg, Julius-von-Sachs-Platz 2, 97082, Würzburg, Germany., Weigel D; Department of Molecular Biology, Max-Planck-Institute for Developmental Biology, Max-Planck-Str. 5, D-72076, Tübingen, Germany., Gust AA; Department of Plant Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany., Nürnberger T; Department of Plant Biochemistry, Centre for Plant Molecular Biology, Eberhard Karls University Tübingen, Auf der Morgenstelle 32, D-72076, Tübingen, Germany.; Department of Biochemistry, University of Johannesburg, Auckland Park, South Africa.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2019 Mar; Vol. 221 (4), pp. 2080-2095. Date of Electronic Publication: 2018 Oct 25.
DOI: 10.1111/nph.15497
Abstrakt: Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine-rich repeat receptor kinases (LRR-RK) FLS2 and EFR, and the LRR receptor protein (LRR-RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR-RK and LRR-RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP-mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23-regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2-signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP-type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.
(© 2018 The Authors New Phytologist © 2018 New Phytologist Trust.)
Databáze: MEDLINE
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