Oxythiamine improves antifungal activity of ketoconazole evaluated in canine Malassezia pachydermatis strains.

Autor: Siemieniuk M; Department of Cytobiochemistry, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15?245, Bialystok, Poland.; Laboratory of Tissue Culture, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15?245, Bialystok, Poland., Sosnowska K; Department of Pharmaceutical Technology, Medical University of Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland., Czerniecki J; Department of Biology and Pathology of Human Reproduction, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland., Czyzewska U; Department of Cytobiochemistry, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15?245, Bialystok, Poland.; Laboratory of Tissue Culture, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15?245, Bialystok, Poland., Winnicka K; Department of Pharmaceutical Technology, Medical University of Bialystok, Mickiewicza 2c, 15-222, Bialystok, Poland., Tylicki A; Department of Cytobiochemistry, Institute of Biology, University of Bialystok, Ciolkowskiego 1J, 15?245, Bialystok, Poland.
Jazyk: angličtina
Zdroj: Veterinary dermatology [Vet Dermatol] 2018 Dec; Vol. 29 (6), pp. 476-e160. Date of Electronic Publication: 2018 Sep 24.
DOI: 10.1111/vde.12688
Abstrakt: Background: Malassezia pachydermatis is an opportunistic yeast involved in skin and ear canal infections of dogs and cats. Reports suggest that strains of M. pachydermatis resistant to commonly used antifungal agents may be emerging. Therefore, new therapeutic strategies should be explored.
Objectives: The synergistic effect of oxythiamine (OT) and ketoconazole (KTC) was analysed using a reference strain and field isolates (n = 66) of M. pachydermatis. Hydrogel formulations containing these components also were evaluated.
Methods and Materials: The minimum inhibitory concentrations (MICs) and minimum fungicidal concentrations (MFCs) of OT, KTC and their mixtures were determined by a broth macrodilution method. The antifungal effects of hydrogel formulations were determined by a plate diffusion method.
Results: The MIC and MFC values of OT were in the range 0.08 × 10 3 to 10 × 10 3  mg/L. All M. pachydermatis strains showed higher susceptibility to KTC (MICs and MFCs in the range 0.04-0.32 mg/L). Formulations that combined OT and KTC showed a synergistic effect for all tested isolates (n = 66). Hydrogels that contained OT at a concentration of 10 × 10 3 or 20 × 10 3  mg/L and KTC at the concentration of 0.1 × 10 3  mg/L showed a stronger effect than a commercially available product with KTC alone (20 × 10 3  mg/L).
Conclusions and Clinical Importance: Synergy of these drugs may allow for successful topical treatment which utilizes lower doses of KTC without changing its therapeutic effectiveness. Hydrogel formulations proved to be attractive drug carriers for potential topical use.
(© 2018 ESVD and ACVD.)
Databáze: MEDLINE
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