A Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases.

Autor: Inci F; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Ozen MO; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Saylan Y; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Miansari M; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Cimen D; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Dhara R; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Chinnasamy T; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Yuksekkaya M; Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston MA 02115 USA., Filippini C; Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston MA 02115 USA., Kumar DK; Department of Medicine Brigham and Women's Hospital Harvard Medical School Boston MA 02115 USA., Calamak S; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Yesil Y; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA., Durmus NG; Department of Biochemistry Stanford University Stanford Genome Technology Center Palo Alto CA 94304 USA., Duncan G; Crime Laboratory Broward County Sheriff's Office Fort Lauderdale FL 33301 USA., Klevan L; DxNow Inc. Gaithersburg MD 20879 USA., Demirci U; Bio-Acoustic MEMS in Medicine (BAMM) Laboratory Canary Center at Stanford for Cancer Early Detection Department of Radiology Stanford School of Medicine Stanford University Palo Alto CA 94304 USA.; Department of Electrical Engineering (by courtesy) Stanford University Stanford CA 94305 USA.
Jazyk: angličtina
Zdroj: Advanced science (Weinheim, Baden-Wurttemberg, Germany) [Adv Sci (Weinh)] 2018 Jun 15; Vol. 5 (9), pp. 1800121. Date of Electronic Publication: 2018 Jun 15 (Print Publication: 2018).
DOI: 10.1002/advs.201800121
Abstrakt: One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis X ), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epithelial fraction. Captured sperm are then lysed on-chip and sperm DNA is isolated. This method reduces assay-time from 8 h to 80 min, providing an inexpensive alternative to current differential extraction techniques, accelerating identification of suspects and advancing public safety.
Databáze: MEDLINE
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