Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk.

Autor:	Bilgiç F; Department of Internal Medicine, Celal Bayar University School of Medicine, Manisa, Turkey., Gerçeker E; Department of Gastroenterology, Gazi Hospital, İzmir, Turkey., Boyacıoğlu SÖ; Department of Medical Genetics, Adnan Menderes University School of Medicine, Aydın, Turkey., Kasap E; Department of Gastroenterology, Celal Bayar University School of Medicine, Manisa, Turkey., Demirci U; Department of Internal Medicine, Celal Bayar University School of Medicine, Manisa, Turkey., Yıldırım H; Department of Medical Biology, Celal Bayar University School of Medicine, Manisa, Turkey., Baykan AR; Department of Gastroenterology, Erzurum Regional Training and Research Hospital, Erzurum, Turkey., Yüceyar H; Department of Gastroenterology, Celal Bayar University School of Medicine, Manisa, Turkey.
Jazyk:	angličtina
Zdroj:	The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology [Turk J Gastroenterol] 2018 Jul; Vol. 29 (4), pp. 427-435.
DOI:	10.5152/tjg.2018.17350
Abstrakt:	Background/aims: Atrophic gastritis (AG), intestinal metaplasia (IM), and Helicobacter pylori (HP) are the risk factors for the development of gastric cancer (GC). Chromatin remodeling is one of the epigenetic mechanisms involved in the carcinogenesis of GC. The purpose of this study was to investigate the expression profiles of defined chromatin remodeling genes in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. Materials and Methods: In total, 95 patients were included in the study. Patients were divided into 3 groups as: GC group (n=34), AG group (n=36), and control group (n=25). AG group was further divided into subgroups based on the presence of HP and IM in gastric mucosa. Chromatin remodeling gene expressions were analyzed using real-time PCR (RT-PCR) array in all groups. Data were evaluated using the RT-qPCR primer assay data analysis software. Results: EED, CBX3, and MTA1 were more overexpressed, whereas ARID1A, ING5, and CBX7 were more underexpressed in the AG and GC groups compared with the controls. No significant differences were observed between the AG and GC groups concerning the expression of these 6 genes, although the fold change levels of these genes in the GC group were well above than in the AG group. EED, CBX3, and MTA1 were significantly more overexpressed in HP- and IM-positive AG subgroup compared with the HP- or IM-negative AG subgroup. Conclusion: In conclusion, our results provide an evidence of epigenetic alterations in AG. Expressions of EED, CBX3, MTA1, ARID1A, ING5, and CBX7 may be considered as promising markers to be used in GC screening for patients with AG.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu