Oryza sativa class III peroxidase (OsPRX38) overexpression in Arabidopsis thaliana reduces arsenic accumulation due to apoplastic lignification.

Autor: Kidwai M; CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India; Integral University, Kursi road, Lucknow, Uttar Pradesh, India., Dhar YV; CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India; Academy of Scientific and Innovative Research, New Delhi, India., Gautam N; CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India; Academy of Scientific and Innovative Research, New Delhi, India., Tiwari M; CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India., Ahmad IZ; Integral University, Kursi road, Lucknow, Uttar Pradesh, India., Asif MH; CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India; Academy of Scientific and Innovative Research, New Delhi, India., Chakrabarty D; CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India; Academy of Scientific and Innovative Research, New Delhi, India. Electronic address: debasis1972@rediffmail.com.
Jazyk: angličtina
Zdroj: Journal of hazardous materials [J Hazard Mater] 2019 Jan 15; Vol. 362, pp. 383-393. Date of Electronic Publication: 2018 Sep 12.
DOI: 10.1016/j.jhazmat.2018.09.029
Abstrakt: ClassIII peroxidases are multigene family of plant-specific peroxidase enzyme. They are involved in various physiological and developmental processes like auxin catabolism, cell metabolism, various biotic, abiotic stresses and cell elongation. In the present study, we identified a class III peroxidase (OsPRX38) from rice which is upregulated several folds in both arsenate (AsV) and arsenite (AsIII) stresses. The overexpression of OsPRX38 in Arabidopsis thaliana significantly enhances Arsenic (As) tolerance by increasing SOD, PRX GST activity and exhibited low H 2 O 2, electrolyte leakage and malondialdehyde content. OsPRX38 overexpression also affect the plant growth by increasing total biomass and seeds production in transgenics than WT under As stress condition. Confocal microscopy revealed that the OsPRX38-YFP fusion protein was localized to the apoplast of the onion epidermal cells. In addition, lignification was positively correlated with an increase in cell-wall-associated peroxidase activities in transgenic plants. This study indicates the role of OsPRX38 in lignin biosynthesis, where lignin act as an apoplastic barrier for As entry in root cells leading to reduction of As accumulation in transgenic. Overall the study suggests that overexpression of OsPRX38 in Arabidopsis thaliana activates the signaling network of different antioxidant systems under As stress condition, enhancing the plant tolerance by reducing As accumulation due to high lignification.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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