An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites.

Autor: Patil SB; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom. Electronic address: samadhan.patil@glasgow.ac.uk., Dheeman DS; Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow G12 8TA, United Kingdom., Al-Rawhani MA; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom., Velugotla S; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom., Nagy B; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom., Cheah BC; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom., Grant JP; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom., Accarino C; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom., Barrett MP; Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow G12 8TA, United Kingdom., Cumming DRS; Electronics and Nanoscale Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, United Kingdom. Electronic address: david.cumming.2@glasgow.ac.uk.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2018 Dec 30; Vol. 122, pp. 88-94. Date of Electronic Publication: 2018 Sep 05.
DOI: 10.1016/j.bios.2018.09.013
Abstrakt: Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.
(Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: