Lignins isolated from Prickly pear cladodes of the species Opuntia fícus-indica (Linnaeus) Miller and Opuntia cochenillifera (Linnaeus) Miller induces mice splenocytes activation, proliferation and cytokines production.

Autor: da Cruz Filho IJ; Laboratory of Biothecnological Process, Antibiotics Department, Federal University of Pernambuco, Pernambuco, Brazil., da Silva Barros BR; Laboratory of Immunological and Antitumor Analyzes, Antibiotics Department, Federal University of Pernambuco, Pernambuco, Brazil., de Souza Aguiar LM; Laboratory of Immunological and Antitumor Analyzes, Antibiotics Department, Federal University of Pernambuco, Pernambuco, Brazil., Navarro CDC; Laboratory of Bioenergetic, Department of Clinical Patology, Campinas State University, São Paulo, Brazil., Ruas JS; Laboratory of Bioenergetic, Department of Clinical Patology, Campinas State University, São Paulo, Brazil., de Lorena VMB; Laboratory of Immunoparasitology, Aggeu Magalhães Institute, FIOCRUZ, Pernambuco, Brazil., de Moraes Rocha GJ; National Laboratory of Bioethanol Science and Technology, National Center for Research in Energy and Materials, São Paulo, Brazil., Vercesi AE; Laboratory of Bioenergetic, Department of Clinical Patology, Campinas State University, São Paulo, Brazil., de Melo CML; Laboratory of Bioenergetic, Department of Clinical Patology, Campinas State University, São Paulo, Brazil. Electronic address: crismout_melo@hotmail.com., Maior AMS; Laboratory of Biothecnological Process, Antibiotics Department, Federal University of Pernambuco, Pernambuco, Brazil.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Feb 15; Vol. 123, pp. 1331-1339. Date of Electronic Publication: 2018 Sep 21.
DOI: 10.1016/j.ijbiomac.2018.09.120
Abstrakt: Opuntia fícus-indica and Opuntia cochenillifera are species of Cactaceae, found in the arid regions of the planet. They present water, cellulose, hemicellulose, pectins, extractives, ashes and lignins. Here we aimed to study the immunomodulatory action of lignins from these two species against mice splenocytes, since no study for this purpose has yet been reported. The antioxidant activities of these lignins were evaluated by the DPPH, ABTS, NO assays and total antioxidant activity. Cytotoxicity was evaluated through Annexin V-FITC and propidium iodide-PE probs and cell proliferation was determined by CFSE. Immunomodulation studies with Opuntia lignins obtained were performed through investigation of ROS levels, cytosolic calcium release, changes on mitochondrial membrane potential, cytokine production and NO release. Results showed that Opuntia cochenillifera lignin presented more phenolic amount and antioxidant activities than Opuntia ficius-indica. Both lignins showed high cell viability (>96%) and cell proliferation. Activation signal was observed for both lignins with increase of ROS and cytosolic calcium levels, and changes in mitochondrial membrane potential. In addition, lignins induced high TNF-α, IL-6 and IL-10 production and reduced NO release. Therefore, these lignins present great potential to be used as molecules with a proinflammatory profile, being shown as a promising therapeutic agent.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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