Sexually Transmitted Infections Detected by Multiplex Real Time PCR in Asymptomatic Women and Association with Cervical Intraepithelial Neoplasia.
| Autor: | Lima LM; Instituto de Educação e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil., Hoelzle CR; Instituto de Educação e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil., Simões RT; Instituto de Educação e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil., Lima MIM; Instituto de Educação e Pesquisa da Santa Casa, Belo Horizonte, MG, Brazil., Fradico JRB; Research and Development Division, Grupo Hermes Pardini, Vespasiano, MG, Brazil., Mateo ECC; Research and Development Division, Grupo Hermes Pardini, Vespasiano, MG, Brazil., Zauli DAG; Research and Development Division, Grupo Hermes Pardini, Vespasiano, MG, Brazil., Melo VH; Faculty of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia [Rev Bras Ginecol Obstet] 2018 Sep; Vol. 40 (9), pp. 540-546. Date of Electronic Publication: 2018 Sep 19. |
| DOI: | 10.1055/s-0038-1669994 |
| Abstrakt: | Objective: To determine the frequency of sexually transmitted infections (STIs) in asymptomatic women and the association of STIs with cervical intraepithelial neoplasia (CIN). Methods: A cross-sectional study was performed, enrolling women examined in a general gynecology clinic and in a colposcopy referral center from October 2014 to October 2015. The colposcopy group consisted of 71 women, and the general gynecology group consisted of 55 women. Cervical samples were collected for cervical cytology and a multiplex real-time polymerase chain reaction (PCR) was developed to detect human papillomavirus (HPV) and the STIs caused by the following microorganisms: Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum , and Neisseria gonorrhoeae . A multivariate analysis was performed by logistic regression, considering the significance level of 0.05. Results: The general frequency of STIs was: 46.8% (HPV); 27.8% ( C. trachomatis ); 28.6% ( M. genitalium ); 0.8% ( M. hominis ); 4.8% ( U. urealyticum ); and 4.8% ( N. gonorrhoeae ). The significant risk factors for CIN were: HPV infection (odds ratio [OR] = 2.53; p = 0.024); C. trachomatis (OR = 3.04; p = 0.009); M. genitalium (OR = 2.37; p = 0.04); and HPV and C. trachomatis coinfection (OR = 3.11; p = 0.023). After the multivariate analysis, a significant association was found between HPV and CIN (OR = 2.48; 95% confidence interval [95%CI]: 1.04-5.92; p = 0.04); and between C. trachomatis and CIN (OR = 2.69; 95%CI: 1.11-6.53; p = 0.028). Conclusion: The frequency of STIs was high in asymptomatic patients. Infections by HPV and C. trachomatis were independently associated with the presence of CIN. The high frequency of STIs in asymptomatic women suggests the need for routine screening of these infections. Competing Interests: The authors have none to declare. (Thieme Revinter Publicações Ltda Rio de Janeiro, Brazil.) |
| Databáze: | MEDLINE |
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