MicroRNA-791 is an independent prognostic factor of papillary thyroid carcinoma and inhibits the proliferation of PTC cells.
| Autor: | Gao XB; Department of Surgery for Vascular Thyroid and Hernia, Xuzhou Central Hospital, Xuzhou, China. jiagaolei@163.com., Chen CL, Tian ZL, Yuan FK, Jia GL |
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| Jazyk: | angličtina |
| Zdroj: | European review for medical and pharmacological sciences [Eur Rev Med Pharmacol Sci] 2018 Sep; Vol. 22 (17), pp. 5562-5568. |
| DOI: | 10.26355/eurrev_201809_15819 |
| Abstrakt: | Objective: To investigate the significance and possible mechanism of miR-791 in the pathogenesis of papillary thyroid carcinoma (PTC). Patients and Methods: The expression of miR-791 in 80 cases of thyroid carcinoma tissues and 80 cases of paracancerous tissues was detected by quantitative Real-time-polymerase chain reaction (qRT-PCR). After miR-791 mimics were transfected into thyroid cancer cells by liposome method, the cell proliferation was detected by Cell Counting Kit-8 (CCK-8) and 5-ethynyl-2'-deoxyuridine (EDU), respectively. Cell cycle was detected by flow cytometry. Results: The expression of miR-791 in thyroid cancer tissue was significantly lower than that of normal thyroid. The mir-719 expression is positively correlated with the prognosis of thyroid carcinoma. After transfection of miR-791 mimics, the proliferation ability of TPC-1 and HTH83 cells was weakened, and the cell cycle was blocked in the G0/G1 phase. Further study on the underlying mechanism found that after overexpression of miR-791, the expressions of Cyclin D1, CKD6 and CDK4 decreased significantly, while the expression of cyclin inhibitor P21 increased significantly. Conclusions: MiR-791 is lowly expressed in thyroid cancer. MiR-791 may inhibit thyroid cancer cell proliferation by blocking thyroid cancer cells in G0/G1 phase, thus participating in the impediment of thyroid cancer development. |
| Databáze: | MEDLINE |
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