Unimicellar hyperstars as multi-antigen cancer nanovaccines displaying clustered epitopes of immunostimulating peptides.

Autor: Kakwere H; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA and Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, CA 94305, USA. kwferrara@ucdavis.edu kwferrar@stanford.edu., Ingham ES; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA., Allen R; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA., Mahakian LM; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA., Tam SM; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA., Zhang H; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA and Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, CA 94305, USA. kwferrara@ucdavis.edu kwferrar@stanford.edu., Silvestrini MT; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA., Lewis JS; Department of Biomedical Engineering, University of California (Davis), Davis, CA 95616, USA., Ferrara KW; Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford University, Stanford, CA 94305, USA. kwferrara@ucdavis.edu kwferrar@stanford.edu.
Jazyk: angličtina
Zdroj: Biomaterials science [Biomater Sci] 2018 Nov 01; Vol. 6 (11), pp. 2850-2858. Date of Electronic Publication: 2018 Sep 19.
DOI: 10.1039/c8bm00891d
Abstrakt: Unimicellar hyperstar macromolecular chimeras displaying multiple melanoma peptide antigens were prepared primarily via a combination of click chemistry and esterification reactions starting from a biodegradable hyperbranched polymer template. Solubilization of the hyperstars in aqueous solution afforded a multi-antigen unimicellar cancer nanovaccine of about 20 nm. The nanovaccine showed good biocompatibility and uptake by dendritic cells in vitro. An in vivo evaluation of the nanovaccine therapeutic efficacy against melanoma in mice implanted with B16OVA tumors revealed significantly greater T-cell recruitment and improved survival rates for mice treated with nanovaccine and adjuvant compared to non-treated mice.
Databáze: MEDLINE
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