A critical evaluation of the use of gas chromatography- and high performance liquid chromatography-mass spectrometry techniques for the analysis of microbial metabolites in human urine after consumption of orange juice.

Autor: Ordóñez JL; Department of Food Science and Health, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Avenida Menéndez-Pidal, SN, 14004, Córdoba, Spain., Pereira-Caro G; Department of Food Science and Health, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Avenida Menéndez-Pidal, SN, 14004, Córdoba, Spain. Electronic address: mariag.pereira@juntadeandalucia.es., Ludwig I; Department of Food and Technology, Universidad de Lleida, 25198, Lleida, Spain., Muñoz-Redondo JM; Department of Food Science and Health, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Avenida Menéndez-Pidal, SN, 14004, Córdoba, Spain., Ruiz-Moreno MJ; Department of Food Science and Health, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Avenida Menéndez-Pidal, SN, 14004, Córdoba, Spain., Crozier A; School of Medicine, Dentistry and Nursing, University of Glasgow, Glasgow G12 8QQ, UK; Department of Nutrition, University of California, Davis, CA 95616-5270, USA., Moreno-Rojas JM; Department of Food Science and Health, Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), Avenida Menéndez-Pidal, SN, 14004, Córdoba, Spain.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2018 Nov 09; Vol. 1575, pp. 100-112. Date of Electronic Publication: 2018 Sep 09.
DOI: 10.1016/j.chroma.2018.09.016
Abstrakt: The present study compared and validated two analytical methods, HPLC-HRMS, and GC-MS using MSTFA as derivatization agent, for the analysis of microbiota-derived phenolic acids and aromatic compounds accumulating in urine, collected over a 24 h period after the consumption of 500 mL of orange juice. In addition, purification procedures using SDB-L and HLB solid phase cartridges were compared when HPLC-HRMS technique was used. Both HPLC-HRMS and GC-MS methodologies were successfully validated in terms of specificity, sensitivity, limit of detection and quantification, recovery and matrix effects. HPLC-HRMS, unlike GC-MS, does not require sample derivatization prior to analysis. GC-MS was not suitable for the analysis of phenolic sulfate and glucuronide metabolites because of their lack of volatility. These phase II metabolites could, however, be analysed by HPLC-HRMS which, as a consequence, provided more detailed and complete information on the phenolic compounds derived from microbiota-mediated degradation of orange juice (poly)phenols. Furthermore, the use of SDB-L and HLB cartridges for sample purification prior to HPLC-HRMS analysis is suitable for free phenolics and glucuronide metabolites but not sulfate derivatives. These findings highlight that the use of an inappropriate analytical protocol can adversely affect studies on the bioavailability of dietary (poly)phenols in which microbiota-derived phenolic catabolites play an important role.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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