Role of β Cell Precursors in the Regeneration of Insulin-Producing Pancreatic β Cells under the Influence of Glucagon-Like Peptide 1.

Autor: Skurikhin EG; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia. eskurikhin@inbox.ru., Pakhomova AV; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Epanchintsev AA; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Stronin OV; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Ermakova NN; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Pershina OV; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Ermolaeva LA; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Krupin VA; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Kudryashova AI; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Zhdanov VV; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia., Dygai AM; Laboratory of Regenerative Pharmacology, E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk Research Medical Center, Russian Academy of Sciences, Tomsk, Russia.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2018 Sep; Vol. 165 (5), pp. 644-648. Date of Electronic Publication: 2018 Sep 17.
DOI: 10.1007/s10517-018-4232-5
Abstrakt: The effects of the pegylated form of glucagon-like peptide 1 (pegGLP-1) on oligopotent β cell precursors (CD45 - TER119 - CD133 + CD49f low ) in the pancreas were studied in C57Bl/6 mice. Under conditions of streptozotocin-induced type 1 diabetes mellitus, intraperitoneal injection of pegGLP1 increased the content of β cell precursors and dithizone-stained cells in the pancreas. β Cell precursors of mice with diabetes demonstrated high self-maintenance potential. In contrast to pegGLP-1, native GLP-1 did not affect β cell precursors in diabetic animals. Treatment of a culture of β cell precursors from mice with diabetes induced the yield of dithizone-stained mononuclears. In conditioned mediums of dithizone-positive cells obtained as a result of differentiation of β cell precursors from mice with diabetes, insulin was detected after administration of pegGLP-1 (10 -7 M) and glucose (3 mmol/liter); the level of insulin increased with increasing glucose concentration (to 20 mmol/liter). The in vitro effect of pegGLP-1 did not differ from the effect of GLP-1 (10 -7 M).
Databáze: MEDLINE
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